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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX81169 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX81169, RRID:AB_11177497
- Product name
- alpha 2b Adrenergic Receptor antibody, Internal
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of ADRA2B Antibody (Center) (GTX81169) in MDA-MB231 cell line lysates (35ug/lane). ADRA2B (arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of ADRA2B Antibody (Center)(GTX81169) with MDA-MB231 cell followed by Alexa Fluor? 488-conjμgated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma with ADRA2B Antibody (Center)(GTX81169), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MDA-231 cells using ADRA2B Antibody (Center)(bottom histogram)(GTX81169) compared to a negative control cell (top histogram). FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
