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Western blot
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NB100-60417 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-60417, RRID:AB_2080191
- Product name
- Rabbit Polyclonal CHD8 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Common CHD8 Genomic Targets Contrast With Model-Specific Transcriptional Impacts of CHD8 Haploinsufficiency.
Chd8 Mutation Leads to Autistic-like Behaviors and Impaired Striatal Circuits.
CHD8 regulates neurodevelopmental pathways associated with autism spectrum disorder in neural progenitors.
Wade AA, Lim K, Catta-Preta R, Nord AS
Frontiers in molecular neuroscience 2018;11:481
Frontiers in molecular neuroscience 2018;11:481
Chd8 Mutation Leads to Autistic-like Behaviors and Impaired Striatal Circuits.
Platt RJ, Zhou Y, Slaymaker IM, Shetty AS, Weisbach NR, Kim JA, Sharma J, Desai M, Sood S, Kempton HR, Crabtree GR, Feng G, Zhang F
Cell reports 2017 Apr 11;19(2):335-350
Cell reports 2017 Apr 11;19(2):335-350
CHD8 regulates neurodevelopmental pathways associated with autism spectrum disorder in neural progenitors.
Sugathan A, Biagioli M, Golzio C, Erdin S, Blumenthal I, Manavalan P, Ragavendran A, Brand H, Lucente D, Miles J, Sheridan SD, Stortchevoi A, Kellis M, Haggarty SJ, Katsanis N, Gusella JF, Talkowski ME
Proceedings of the National Academy of Sciences of the United States of America 2014 Oct 21;111(42):E4468-77
Proceedings of the National Academy of Sciences of the United States of America 2014 Oct 21;111(42):E4468-77
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CHD8 Antibody [NB100-60417] - Detection of Mouse CHD8 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-CHD8 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CHD8 Antibody [NB100-60417] - Detection of Human CHD8 on HeLa whole cell lysate (5, 15, and 50 microgram) using NB100-60417. CHD8 was also immunoprecipitated by rabbit anti-CHD8 antibodies BL5037 and NB100-60418.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CHD8 Antibody [NB100-60417] - Detection of Human and Mouse CHD8 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CHD8 antibody NB100-60417 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CHD8 Antibody [NB100-60417] - Detection of human CHD8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD8 antibody NB100-60417 used for IP at 6 ug/mg lysate. CHD8 was also immunoprecipitated by rabbit anti-CHD8 antibody NB100-60418. For blotting immunoprecipitated CHD8, NB100-60417 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CHD8 Antibody [NB100-60417] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CHD8 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
