PAB21403

antibody from Abnova Corporation

Targeting: MYO19 FLJ22865, MYOHD1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21403

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21403, RRID:AB_10962062

Product name

MYO19 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant MYO19.

Antigen sequence

HNPGSDGQAREYLREDLQEFLGGEVLLYKLDDLTR
VNPVTLETVLRCLQARYMADTFYTNAGCTLVALNP
FKPVPQLYSPELMREYHAAPQPQKLKPHV


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with MYO19 polyclonal antibody (Cat # PAB21403).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251MG with MYO19 polyclonal antibody (Cat # PAB21403) at 1-4 ug/mL dilution shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach with MYO19 polyclonal antibody (Cat # PAB21403) shows moderate cytoplasmic positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)