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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-967 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-967, RRID:AB_628249
- Product name
- Anti-SHC1
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references Friend of Prmt1, a novel chromatin target of protein arginine methyltransferases.
SHIP prevents lipopolysaccharide from triggering an antiviral response in mice.
A low concentration of genistein induces estrogen receptor-alpha and insulin-like growth factor-I receptor interactions and proliferation in uterine leiomyoma cells.
The role of Shc and insulin-like growth factor 1 receptor in mediating the translocation of estrogen receptor alpha to the plasma membrane.
SHIP-mediated inhibition of K562 erythroid differentiation requires an intact catalytic domain and Shc binding site
Accelerated mammary tumor development in mutant polyomavirus middle T transgenic mice expressing elevated levels of either the Shc or Grb2 adapter protein.
van Dijk TB, Gillemans N, Stein C, Fanis P, Demmers J, van de Corput M, Essers J, Grosveld F, Bauer UM, Philipsen S
Molecular and cellular biology 2010 Jan;30(1):260-72
Molecular and cellular biology 2010 Jan;30(1):260-72
SHIP prevents lipopolysaccharide from triggering an antiviral response in mice.
Sly LM, Hamilton MJ, Kuroda E, Ho VW, Antignano FL, Omeis SL, van Netten-Thomas CJ, Wong D, Brugger HK, Williams O, Feldman ME, Houseman BT, Fiedler D, Shokat KM, Krystal G
Blood 2009 Mar 26;113(13):2945-54
Blood 2009 Mar 26;113(13):2945-54
A low concentration of genistein induces estrogen receptor-alpha and insulin-like growth factor-I receptor interactions and proliferation in uterine leiomyoma cells.
Di X, Yu L, Moore AB, Castro L, Zheng X, Hermon T, Dixon D
Human reproduction (Oxford, England) 2008 Aug;23(8):1873-83
Human reproduction (Oxford, England) 2008 Aug;23(8):1873-83
The role of Shc and insulin-like growth factor 1 receptor in mediating the translocation of estrogen receptor alpha to the plasma membrane.
Song RX, Barnes CJ, Zhang Z, Bao Y, Kumar R, Santen RJ
Proceedings of the National Academy of Sciences of the United States of America 2004 Feb 17;101(7):2076-81
Proceedings of the National Academy of Sciences of the United States of America 2004 Feb 17;101(7):2076-81
SHIP-mediated inhibition of K562 erythroid differentiation requires an intact catalytic domain and Shc binding site
Josie Siegel, Yun Li, Peter Whyte
Oncogene 1999 Nov;18(50):7135-7148
Oncogene 1999 Nov;18(50):7135-7148
Accelerated mammary tumor development in mutant polyomavirus middle T transgenic mice expressing elevated levels of either the Shc or Grb2 adapter protein.
Rauh MJ, Blackmore V, Andrechek ER, Tortorice CG, Daly R, Lai VK, Pawson T, Cardiff RD, Siegel PM, Muller WJ
Molecular and cellular biology 1999 Dec;19(12):8169-79
Molecular and cellular biology 1999 Dec;19(12):8169-79
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 63
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
