AM20940PU-N

antibody from OriGene

Targeting: SHC1 p66, SHC, ShcA

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Data presented on provider website)

Antibody Data

Product number

AM20940PU-N

Provider

OriGene

Product name

SHC (SHC1) mouse monoclonal antibody, clone 3F4

Antibody type

Monoclonal

Description

SHC (SHC1) mouse monoclonal antibody, clone 3F4

Host

Mouse

Conjugate

Unconjugated

Epitope

SHC1

Antibody clone number

3F4

Vial size

50 µg

Concentration

0.35 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

SHC1 monoclonal antibody clone 3F4 Western Blot analysis of SHC1 expression in A-431.

Validation comment

WB

ELISA

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SHC1 is approximately 0.03 ng/ml as a capture antibody.

Validation comment

ELISA

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Proximity Ligation Analysis (PLA) of protein-protein interactions between PIK3R1 and SHC1 HeLa cells were stained with anti-PIK3R1 rabbit purified polyclonal 1/1200 and anti-SHC1 mouse monoclonal antibody 1/50.

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to SHC1 on A-431 cell (antibody concentration 10 ug/ml).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Human Colon, myenteric plexus: Formalin-Fixed, Paraffin-Embedded (FFPE)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Human Adipocytes: Formalin-Fixed, Paraffin-Embedded (FFPE)

Validation comment

IHC