TA501056

antibody from OriGene

Targeting: SHC1 p66, SHC, ShcA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501056

Provider

OriGene

Proper citation

OriGene Cat#TA501056, RRID:AB_11126188

Product name

Anti-SHC1 (SHC) mouse monoclonal antibody, clone OTI3A1 (formerly 3A1)

Antibody type

Monoclonal

Description

Anti-SHC1 (SHC) mouse monoclonal antibody, clone OTI3A1 (formerly 3A1)

Host

Mouse

Conjugate

Unconjugated

Epitope

SHC1

Isotype

IgG

Antibody clone number

OTI3A1

Vial size

100 µl

Concentration

1 mg/ml

References

Submitted references

Role of SNTA1 in Rac1 activation, modulation of ROS generation, and migratory potential of human breast cancer cells.

Bhat HF, Baba RA, Adams ME, Khanday FA
British journal of cancer 2014 Feb 4;110(3):706-14

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHC1 (RC204362, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHC1.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-SHC1 mouse monoclonal antibody (TA501056) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SHC1(RC204362).

Validation comment

IF

Immunoprecipitation

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Figure from citation: Immunoprecipitation of SHC1(p66shc) protein level by using anti-SHC1 antibody in human HBL-100/MCF-7 cell lysates.

Validation comment

IP

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-SHC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501056, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-SHC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501056, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-SHC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501056, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-SHC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501056, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-SHC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501056, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-SHC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501056, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded bladder tissue within the normal limits using anti-SHC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501056, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-SHC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501056, Dilution 1:50)

Validation comment

IHC