A00796-2

antibody from Boster Biological Technology

Targeting: SHC1 p66, SHC, ShcA

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A00796-2

Provider

Boster Biological Technology

Product name

Anti-Shc1 (Ab-427) Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal antibody for P29353 detection. Tested positive for WB IHC IF in Hu

Reactivity

Human

Host

Rabbit

Vial size

100ul

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

Store at -20°C for long term preservation (recommended) . Store at 4°C for short term use.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of SHC1 using anti-SHC1 antibody (A00796-2).SHC1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SHC1 Antibody (A00796-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image