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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-97096 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TEFM Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KSTTPKKITP NVTFCDENAK EPENALDKLF SSEQQASILH VLNTASTKEL EAFRLLRGRR SINIVEHREN FGPFQNLESL MNVPLFKYKS TVQVCNSILC PKTGREKRKS PENRFLRKLL KPDIERERLK AVNSIISIVF GTRRIAWAHL DRKLTVLDWQ QSDRWSLMRG IYSSS; Positive Samples: HL-60, K-562, Mouse liver, Rat liver; Cellular Location: Mitochondrion matrix, mitochondrion nucleoid
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.75 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TEFM in extracts of various cell lines using TEFM Polyclonal Antibody (Product # PA5-97096) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TEFM was performed in U2OS cells using TEFM Polyclonal Antibody (Product # PA5-97096) at a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TEFM in paraffin-embedded rat testis using TEFM Polyclonal Antibody (Product # PA5-97096) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TEFM in paraffin-embedded rat pancreas using TEFM Polyclonal Antibody (Product # PA5-97096) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TEFM in paraffin-embedded human colon carcinoma using TEFM Polyclonal Antibody (Product # PA5-97096) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TEFM in paraffin-embedded mouse spleen using TEFM Polyclonal Antibody (Product # PA5-97096) at a dilution of 1:100.
