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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NB100-79810 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-79810, RRID:AB_1108023
- Product name
- Rabbit Polyclonal CtIP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references ASF1a Promotes Non-homologous End Joining Repair by Facilitating Phosphorylation of MDC1 by ATM at Double-Strand Breaks.
MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex.
CtIP and MRN promote non-homologous end-joining of etoposide-induced DNA double-strand breaks in G1.
RAP80-directed tuning of BRCA1 homologous recombination function at ionizing radiation-induced nuclear foci.
Lee KY, Im JS, Shibata E, Dutta A
Molecular cell 2017 Oct 5;68(1):61-75.e5
Molecular cell 2017 Oct 5;68(1):61-75.e5
MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex.
Lee KY, Im JS, Shibata E, Park J, Handa N, Kowalczykowski SC, Dutta A
Nature communications 2015 Jul 28;6:7744
Nature communications 2015 Jul 28;6:7744
CtIP and MRN promote non-homologous end-joining of etoposide-induced DNA double-strand breaks in G1.
Quennet V, Beucher A, Barton O, Takeda S, Löbrich M
Nucleic acids research 2011 Mar;39(6):2144-52
Nucleic acids research 2011 Mar;39(6):2144-52
RAP80-directed tuning of BRCA1 homologous recombination function at ionizing radiation-induced nuclear foci.
Hu Y, Scully R, Sobhian B, Xie A, Shestakova E, Livingston DM
Genes & development 2011 Apr 1;25(7):685-700
Genes & development 2011 Apr 1;25(7):685-700
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CtIP Antibody [NB100-79810] - Detection of Human CtIP on MCF7 cell lysate (5, 15, and 50 microgram) and HeLa whole cell lysate (50 microgram) using NB100-79810. CtIP was also immunoprecipitated using rabbit anti-CtIP antibody NB100-79809.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CtIP Antibody [NB100-79810] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CtIP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CtIP Antibody [NB100-79810] - Detection of human CtIP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CtIP antibody NB100-79810 used for IP at 3 ug per reaction. CtIP was also immunoprecipitated by rabbit anti-CtIP antibody NB100-79809. For blotting immunoprecipitated CtIP, NB100-79810 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
