15456-1-AP

antibody from Invitrogen Antibodies

Targeting: NOL12 MGC3731, Nop25, RRP17

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15456-1-AP

Provider

Invitrogen Antibodies

Product name

NOL12 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MGRNKKKKR DGDDRRPRLV LSFDEEKRRE YLTGFHKRKV ERKKAAIEEI KQRLKEEQRK LREERHQEYL KMLAEREEAL EEADELDRLV TAKTESVQYD HPNHTVTVTT ISDLDLSGAR LLGLTPPEGG AGDRSEEEAS STEKPTKALP RKSRDPLLSQ RISSLTASLH AHSRKKVKRK HPRRAQDSKK PPRAPRTSKA QRRRLTGKAR HSGE (1-213 aa encoded by BC002808)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.24 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 15456-1-AP (NOL12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-NOL12 (IP:15456-1-AP, 4ug; Detection:15456-1-AP 1:300) with HeLa cells lysate 4000ug.