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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [22]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-278-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL5/RANTES Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CCL5/RANTES in direct ELISAs. Detects human CCL5/RANTES and mouse CCL5/RANTES Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant canine CCL5/RANTES is observed and approximately 20% cross-reactivity with recombinant cotton rat CCL5/RANTES is observed. In Western blots, no cross-reactivity with recombinant human (rh) CCL3 and rhCCL4 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P13501- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references CCR5/CCL5 axis interaction promotes migratory and invasiveness of pancreatic cancer cells.
An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium.
The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive Therapy.
NK cell responses to simian immunodeficiency virus vaginal exposure in naive and vaccinated rhesus macaques.
Lenalidomide inhibits lymphangiogenesis in preclinical models of mantle cell lymphoma.
Expression and regulation of chemokines in murine and human type 1 diabetes.
Irritant-induced migration of Langerhans cells coincides with an IL-10-dependent switch to a macrophage-like phenotype.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
Disrupting functional interactions between platelet chemokines inhibits atherosclerosis in hyperlipidemic mice.
Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways.
Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes.
Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation.
Mesenchymal stem cells within tumour stroma promote breast cancer metastasis.
Renal transplantation: examination of the regulation of chemokine binding during acute rejection.
Renal transplantation: examination of the regulation of chemokine binding during acute rejection.
The beta chemokines CCL4 and CCL5 enhance adhesion of specific CD4+ T cell subsets to human brain endothelial cells.
The beta chemokines CCL4 and CCL5 enhance adhesion of specific CD4+ T cell subsets to human brain endothelial cells.
Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes.
Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity.
Singh SK, Mishra MK, Eltoum IA, Bae S, Lillard JW Jr, Singh R
Scientific reports 2018 Jan 22;8(1):1323
Scientific reports 2018 Jan 22;8(1):1323
An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium.
Rump L, Mattey DL, Kehoe O, Middleton J
Cytokine 2017 Sep;97:133-140
Cytokine 2017 Sep;97:133-140
The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive Therapy.
Zsiros E, Duttagupta P, Dangaj D, Li H, Frank R, Garrabrant T, Hagemann IS, Levine BL, June CH, Zhang L, Wang E, Marincola FM, Bedognetti D, Powell DJ Jr, Tanyi J, Feldman MD, Kandalaft LE, Coukos G
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Jun 15;21(12):2840-50
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Jun 15;21(12):2840-50
NK cell responses to simian immunodeficiency virus vaginal exposure in naive and vaccinated rhesus macaques.
Shang L, Smith AJ, Duan L, Perkey KE, Qu L, Wietgrefe S, Zupancic M, Southern PJ, Masek-Hammerman K, Reeves RK, Johnson RP, Haase AT
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 1;193(1):277-84
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 1;193(1):277-84
Lenalidomide inhibits lymphangiogenesis in preclinical models of mantle cell lymphoma.
Song K, Herzog BH, Sheng M, Fu J, McDaniel JM, Chen H, Ruan J, Xia L
Cancer research 2013 Dec 15;73(24):7254-64
Cancer research 2013 Dec 15;73(24):7254-64
Expression and regulation of chemokines in murine and human type 1 diabetes.
Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D
Diabetes 2012 Feb;61(2):436-46
Diabetes 2012 Feb;61(2):436-46
Irritant-induced migration of Langerhans cells coincides with an IL-10-dependent switch to a macrophage-like phenotype.
Ouwehand K, Oosterhoff D, Breetveld M, Scheper RJ, de Gruijl TD, Gibbs S
The Journal of investigative dermatology 2011 Feb;131(2):418-25
The Journal of investigative dermatology 2011 Feb;131(2):418-25
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS
Journal of immunology (Baltimore, Md. : 1950) 2009 Mar 15;182(6):3919-27
Journal of immunology (Baltimore, Md. : 1950) 2009 Mar 15;182(6):3919-27
Disrupting functional interactions between platelet chemokines inhibits atherosclerosis in hyperlipidemic mice.
Koenen RR, von Hundelshausen P, Nesmelova IV, Zernecke A, Liehn EA, Sarabi A, Kramp BK, Piccinini AM, Paludan SR, Kowalska MA, Kungl AJ, Hackeng TM, Mayo KH, Weber C
Nature medicine 2009 Jan;15(1):97-103
Nature medicine 2009 Jan;15(1):97-103
Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways.
Renaud SJ, Sullivan R, Graham CH
Placenta 2009 Apr;30(4):313-9
Placenta 2009 Apr;30(4):313-9
Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes.
Chou SY, Weng JY, Lai HL, Liao F, Sun SH, Tu PH, Dickson DW, Chern Y
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Mar 26;28(13):3277-90
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Mar 26;28(13):3277-90
Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation.
Weigt SS, Elashoff RM, Keane MP, Strieter RM, Gomperts BN, Xue YY, Ardehali A, Gregson AL, Kubak B, Fishbein MC, Saggar R, Ross DJ, Lynch JP 3rd, Zisman DA, Belperio JA
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2008 Jul;8(7):1512-22
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2008 Jul;8(7):1512-22
Mesenchymal stem cells within tumour stroma promote breast cancer metastasis.
Karnoub AE, Dash AB, Vo AP, Sullivan A, Brooks MW, Bell GW, Richardson AL, Polyak K, Tubo R, Weinberg RA
Nature 2007 Oct 4;449(7162):557-63
Nature 2007 Oct 4;449(7162):557-63
Renal transplantation: examination of the regulation of chemokine binding during acute rejection.
Ali S, Malik G, Burns A, Robertson H, Kirby JA
Transplantation 2005 Mar 27;79(6):672-9
Transplantation 2005 Mar 27;79(6):672-9
Renal transplantation: examination of the regulation of chemokine binding during acute rejection.
Ali S, Malik G, Burns A, Robertson H, Kirby JA
Transplantation 2005 Mar 27;79(6):672-9
Transplantation 2005 Mar 27;79(6):672-9
The beta chemokines CCL4 and CCL5 enhance adhesion of specific CD4+ T cell subsets to human brain endothelial cells.
Quandt J, Dorovini-Zis K
Journal of neuropathology and experimental neurology 2004 Apr;63(4):350-62
Journal of neuropathology and experimental neurology 2004 Apr;63(4):350-62
The beta chemokines CCL4 and CCL5 enhance adhesion of specific CD4+ T cell subsets to human brain endothelial cells.
Quandt J, Dorovini-Zis K
Journal of neuropathology and experimental neurology 2004 Apr;63(4):350-62
Journal of neuropathology and experimental neurology 2004 Apr;63(4):350-62
Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
Journal of leukocyte biology 2003 May;73(5):584-90
Journal of leukocyte biology 2003 May;73(5):584-90
Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
Journal of leukocyte biology 2003 May;73(5):584-90
Journal of leukocyte biology 2003 May;73(5):584-90
Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes.
Binninger-Schinzel D, Norley S, Adler HS, Oberg HH, Kurth R
Virology 2002 Mar 1;294(1):209-21
Virology 2002 Mar 1;294(1):209-21
Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity.
Sato E, Koyama S, Robbins RA
The European respiratory journal 2000 Nov;16(5):951-8
The European respiratory journal 2000 Nov;16(5):951-8
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Recombinant Human and Mouse CCL5/RANTES by Western Blot. Western blot shows 25 ng of Recombinant Human CCL5/RANTES (Catalog # 278-RN), Recombinant Mouse CCL5/RANTES (Catalog # 478-MR), Recombinant Human CCL3/MIP-1 alpha Isoform LD78a (Catalog # 270-LD), and Recombinant Human CCL4/MIP-1 beta (Catalog # 271-BME). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL5/RANTES at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL5/RANTES in Human PBMCs. CCL5/RANTES was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (orange; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL5/RANTES in Human Tonsil. CCL5/RANTES was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody. Recombinant Human CCL5/RANTES (Catalog # 278-RN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA). The ND50 is typically 0.1-0.4 µg/mL.
