sc-8330

antibody from Santa Cruz Biotechnology

Targeting: IKBKG FIP-3, FIP3, Fip3p, IKK-gamma, IP1, IP2, NEMO, ZC2HC9

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-8330

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-8330, RRID:AB_2124846

Product name

Anti-IKBKG

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

References

Submitted references

Epstein-Barr virus-encoded EBNA1 inhibits the canonical NF-kappaB pathway in carcinoma cells by inhibiting IKK phosphorylation.

Valentine R, Dawson CW, Hu C, Shah KM, Owen TJ, Date KL, Maia SP, Shao J, Arrand JR, Young LS, O'Neil JD
Molecular cancer 2010 Jan 5;9:1

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NEMO-binding domains of both IKKalpha and IKKbeta regulate IkappaB kinase complex assembly and classical NF-kappaB activation.

Solt LA, Madge LA, May MJ
The Journal of biological chemistry 2009 Oct 2;284(40):27596-608

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Inactivation of NF-kappaB p50 leads to insulin sensitization in liver through post-translational inhibition of p70S6K.

Gao Z, Yin J, Zhang J, He Q, McGuinness OP, Ye J
The Journal of biological chemistry 2009 Jul 3;284(27):18368-76

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Inflammation and NFkappaB activation is decreased by hypothermia following global cerebral ischemia.

Webster CM, Kelly S, Koike MA, Chock VY, Giffard RG, Yenari MA
Neurobiology of disease 2009 Feb;33(2):301-12

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Interleukin-1-induced NF-kappaB activation is NEMO-dependent but does not require IKKbeta.

Solt LA, Madge LA, Orange JS, May MJ
The Journal of biological chemistry 2007 Mar 23;282(12):8724-33

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Key function for the Ubc13 E2 ubiquitin-conjugating enzyme in immune receptor signaling

Masahiro Yamamoto, Toru Okamoto, Kiyoshi Takeda, Shintaro Sato, Hideki Sanjo, Satoshi Uematsu, Tatsuya Saitoh, Naoki Yamamoto, Hiroaki Sakurai, Ken J Ishii, Shoji Yamaoka, Taro Kawai, Yoshiharu Matsuura, Osamu Takeuchi, Shizuo Akira
Nature Immunology 2006 Jul;7(9):962-970

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Positive and negative regulation of EAAT2 by NF-kappaB: a role for N-myc in TNFalpha-controlled repression.

Sitcheran R, Gupta P, Fisher PB, Baldwin AS
The EMBO journal 2005 Feb 9;24(3):510-20

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Deletion of IKK2 in hepatocytes does not sensitize these cells to TNF-induced apoptosis but protects from ischemia/reperfusion injury.

Luedde T, Assmus U, Wüstefeld T, Meyer zu Vilsendorf A, Roskams T, Schmidt-Supprian M, Rajewsky K, Brenner DA, Manns MP, Pasparakis M, Trautwein C
The Journal of clinical investigation 2005 Apr;115(4):849-59

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Activation of the NF-kappaB pathway in human cytomegalovirus-infected cells is necessary for efficient transactivation of the major immediate-early promoter.

DeMeritt IB, Milford LE, Yurochko AD
Journal of virology 2004 May;78(9):4498-507

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band corresponding to the predicted size in kDa (+/-20%).

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 219, 111, 83, 48, 32, 26, 17

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 56

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in lymphoid cells outside reaction centra.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.