Explore
Validate
LearnBS2383
antibody from Bioworld Technology, Inc
Targeting: IKBKG
FIP-3, FIP3, Fip3p, IKK-gamma, IP1, IP2, NEMO, ZC2HC9
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- BS2383 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS2383, RRID:AB_1663725
- Product name
- IKKÎÛ (A314) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 280-330 of Human IKKÎÛ.
- Description
- Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase Ï« (IKKÏ«), previously designated CHUK, interacts with IkB-Ï« and specifically phosphorylates I?BÏ« on Serine 32 and 36, the sites that trigger its degradation. IKKÏ« appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKKÏ« is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKÏ«, IKKÉÇ and IKKÎÛ (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of IKK¦Ã (A314) pAb at 1:500 dilutionLane1:HEK293T cell lysateLane2:Hela cell lysateLane3:Raw264.7 cell lysateLane4:NIH-3T3 cell lysateLane5:pc12 cell lysateLane6:Rat heart tissue lysate
