Explore
Validate
LearnH00008517-M01
antibody from Abnova Corporation
Targeting: IKBKG
FIP-3, FIP3, Fip3p, IKK-gamma, IP1, IP2, NEMO, ZC2HC9
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Proximity ligation assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00008517-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00008517-M01, RRID:AB_509397
- Product name
- IKBKG monoclonal antibody (M01), clone 4G9
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant IKBKG.
- Antigen sequence
MNRHLWKSQLCEMVQPSGGPAADQDVLGEESPLGK
PAMLHLPSEQGAPETLQRCLEENQELRDAIRQSNQ
ILRERCEELLHFQASQREEKEFLMCKFQEARKLVE
RLGLEKLDLKRQKEQALREVEHLKRCQQQMAEDKA
SVKAQVTSLLGELQESQSRLEAATKECQALEGRAR
AASEQARQLESEREALQQQHSVQVDQLRMQGQSVE
AALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHI
KSSVVGSERKRGMQLEDLKQQLQQAEEALVAKQEV
IDKLKEEAEQHKIVMETVPVLKAQADIYKADFQAE
RQAREKLAEKKELLQEQLEQLQREYSKLKASCQES
ARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQR
RSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE- Isotype
- IgG
- Antibody clone number
- 4G9
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- IKBKG monoclonal antibody (M01), clone 4G9 Western Blot analysis of IKBKG expression in K-562 ( Cat # L009V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged IKBKG is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to IKBKG on HeLa cell. [antibody concentration 8 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to IKBKG on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between RALBP1 and IKBKG. HeLa cells were stained with anti-RALBP1 rabbit purified polyclonal 1:1200 and anti-IKBKG mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
