A00874

antibody from Boster Biological Technology

Targeting: IKBKG FIP-3, FIP3, Fip3p, IKK-gamma, IP1, IP2, NEMO, ZC2HC9

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A00874

Provider

Boster Biological Technology

Product name

Anti-IKK gamma/IKBKG Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for IKK gamma/IKBKG detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB, ICC/IF, FCM. Reactive species: Human;Mouse;Rat. IKK gamma/IKBKG information: Molecular Weight: 48198 MW; Subcellular Localization: Cytoplasm . Nucleus . Sumoylated NEMO accumulates in the nucleus in response to genotoxic stress; Tissue Specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of IKK gamma using anti-IKK gamma antibody (A00874). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.lane 1: rat cardiac muscle tissue lysates,lane 2: HEPG2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKK gamma antigen affinity purified polyclonal antibody (Catalog # A00874) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKK gamma at approximately 48KD. The expected band size for IKK gamma is at 48KD.

Additional image