10-6017
antibody from ABEOMICS Inc.
Targeting: IKBKG
FIP-3, FIP3, Fip3p, IKK-gamma, IP1, IP2, NEMO, ZC2HC9
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [2]
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Validation data
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- Product number
- 10-6017 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-Ikk Gamma Antibody
- Antibody type
- Monoclonal
- Description
- IKK is a trimeric protein complex consisting of two catalytic subunits, IKKalpha and IKKbeta, and a regulatory subunit, IKKgamma. IKKgamma (also termed NEMO or IKBKG) regulates the kinase activity of IKKalpha/beta. IKKgamma plays a critical role in DNA damaged-induced NF-kappaB signaling in a role independent of its function in the IKK complex. While the biological importance of IKKgamma PTMs (Post-Translational Modifications) has been reported under a variety of stimuli, the detection of PTMs has numerous technical limitations. These limitations include both the transient nature of these modifications, as well as low overall abundance of modified IKKgamma inside the cell.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A full length recombinant Ikk Gamma
protein was used as the immunogen
for this antibody.- Isotype
- IgG
- Antibody clone number
- ABM15D9
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of Ikk Gamma. Anti-Ikk Gamma antibody (Clone: ABM15D9) was tested at 2 µg/ml on human heart lysate.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular flowcytometric analysis of Ikk Gamma in MCF-7 cell line using 0.5 µg/10^6 cells of Anti-Ikk Gamma (Clone: ABM15D9). Green Represent isotype control and red represent Anti-Ikk Gamma (10-6017 Abeomics). Goat Anti-Mouse PE conjugated was used as secondary antibody.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular flowcytometric analysis of Ikk Gamma in HeLa cell line using 0.5 µg/10^6 cells of Anti-Ikk Gamma (Clone: ABM15D9). Green Represent isotype control and red represent Anti-Ikk Gamma (10-6017 Abeomics). Goat Anti-Mouse PE conjugated was used as secondary antibody.
- Protocol
- Protocol