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LearnBSM-54012R
antibody from Invitrogen Antibodies
Targeting: IKBKG
FIP-3, FIP3, Fip3p, IKK-gamma, IP1, IP2, NEMO, ZC2HC9
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Flow cytometry [1]
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- Product number
- BSM-54012R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IKK gamma Monoclonal Antibody (4A4)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 4A4
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Hela cell lysate probed with IKK gamma (4A4) Monoclonal Antibody (bsm-54012R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF(ICC) staining with IKK gamma (4A4) Monoclonal Antibody (bsm-54012R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded human colon cancer tissue incubated with IKK gamma (4A4) Monoclonal Antibody (bsm-54012R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with IKK gamma (4A4) Monoclonal Antibody (bsm-54012R) at 1:30, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Human placenta tissue incubated with IKK gamma (4A4) Monoclonal Antibody (bsm-54012R) at 1:30, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded mouse colon tissue incubated with IKK gamma (4A4) Monoclonal Antibody (bsm-54012R) at 1:30, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded human colon tissue incubated with IKK gamma (4A4) Monoclonal Antibody (bsm-54012R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells with IKK gamma (4A4) Monoclonal Antibody (bsm-54012R) at a 1:50 dilution (red) compared with an unlabeled control (cells with out incubation with primary antibody; black).
