15682-1-AP

antibody from Invitrogen Antibodies

Targeting: MRPL45 MGC11321

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15682-1-AP

Provider

Invitrogen Antibodies

Product name

MRPL45 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: AGLVIPPEK SDRSIHLACT AGIFDAYVPP EGDARISSLS KEGLIERTER MKKTMASQVS IRRIKDYDAN FKIKDFPEKA KDIFIEAHLC LNNSDHDRLH TLVTEHCFPD MTWDIKYKTV RWSFVESLEP SHVVQVRCSS MMNQGNVYGQ ITVRMHTRQT LAIYDRFGRL MYGQEDVPKD VLEYVVFEKQ LTNPYGSWRM HTKIVPPWAP PKQPILKTVM IPGPQLKPEE EYEEAQGEAQ KPQLA (86-329 aa encoded by B C006235)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.16 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human liver tissue were subjected to SDS PAGE followed by western blot with 15682-1-AP (MRPL45 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 15682-1-AP (MRPL45 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 15682-1-AP (MRPL45 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 15682-1-AP (MRPL45 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 15682-1-AP (MRPL45 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tumor using 15682-1-AP (MRPL45 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tumor using 15682-1-AP (MRPL45 antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-MRPL45 (IP:15682-1-AP, 3ug; Detection:15682-1-AP 1:500) with HeLa cells lysate 2000ug.