N5139

antibody from MilliporeSigma / Merck KGaA

Targeting: NEFL CMT1F, CMT2E, NF68, NFL, PPP1R110

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Protein array (Recommended by provider)

Antibody Data

Product number

N5139

Provider

MilliporeSigma / Merck KGaA

Product name

Monoclonal Anti-Neurofilament 68 antibody produced in mouse

Antibody type

Monoclonal

Reactivity

Human, Rat, Chicken/Avian, Porcine

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

NR4

Storage

Store at -20°C

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band corresponding to the predicted size in kDa (+/-20%).

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 219, 111, 83, 48, 32, 26, 17

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 43

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.

Validation comment

Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.