AM06714PU-N

antibody from OriGene

Targeting: NEFL CMT1F, CMT2E, NF68, NFL, PPP1R110

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM06714PU-N

Provider

OriGene

Product name

Neurofilament (NEFL) mouse monoclonal antibody, clone 1H3, Purified

Antibody type

Monoclonal

Description

Neurofilament (NEFL) mouse monoclonal antibody, clone 1H3, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

NEFL

Isotype

IgG

Antibody clone number

1H3

Vial size

100 µg

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis using NEFL mAb against human NEFL (AA: 422-543) recombinant protein. (Expected MW is 62 kDa)

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.

Validation comment

WB

ELISA

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)

Validation comment

ELISA

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescence analysis of Hela cells using NEFL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NEFL mouse mAb with DAB staining.

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using NEFL mouse mAb with DAB staining.

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).

Validation comment

FC