AF5247

antibody from R&D Systems

Targeting: CD24 CD24A

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

AF5247

Provider

R&D Systems

Product name

Human CD24 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human CD24 in direct ELISAs and Western blots.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

NP_037362


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

CD24 regulates cancer stem cell (CSC)-like traits and a panel of CSC-related molecules serves as a non-invasive urinary biomarker for the detection of bladder cancer.

Ooki A, VandenBussche CJ, Kates M, Hahn NM, Matoso A, McConkey DJ, Bivalacqua TJ, Hoque MO
British journal of cancer 2018 Oct;119(8):961-970

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Arsenic promotes the COX2/PGE2-SOX2 axis to increase the malignant stemness properties of urothelial cells.

Ooki A, Begum A, Marchionni L, VandenBussche CJ, Mao S, Kates M, Hoque MO
International journal of cancer 2018 Jul 1;143(1):113-126

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PMP22 Regulates Self-Renewal and Chemoresistance of Gastric Cancer Cells.

Cai W, Chen G, Luo Q, Liu J, Guo X, Zhang T, Ma F, Yuan L, Li B, Cai J
Molecular cancer therapeutics 2017 Jun;16(6):1187-1198

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human CD24 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5247) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD24 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.