GTX128954

antibody from GeneTex

Targeting: H3C1 H3/A, H3FA, HIST1H3A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Immunoelectron microscopy (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX128954

Provider

GeneTex

Proper citation

GeneTex Cat#GTX128954, RRID:AB_2783841

Product name

Histone H3K4me3 (trimethyl Lys4) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat, Zebrafish

Host

Rabbit

Storage

Keep as concentrated solution. Aliquot and store at -20?C or below. Avoid multiple freeze-thaw cycles.

References

Submitted references

The co-existence of transcriptional activator and transcriptional repressor MEF2 complexes influences tumor aggressiveness.

Di Giorgio E, Franforte E, Cefalù S, Rossi S, Dei Tos AP, Brenca M, Polano M, Maestro R, Paluvai H, Picco R, Brancolini C
PLoS genetics 2017 Apr;13(4):e1006752

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Vitamin C promotes pluripotency of human induced pluripotent stem cells via the histone demethylase JARID1A.

Eid W, Abdel-Rehim W
Biological chemistry 2016 Nov 1;397(11):1205-1213

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Histone H3 (tri-Methyl Lys4) antibody detects Histone H3 (tri-Methyl Lys4) protein by Western blot analysis.A. 30 μg HeLa whole cell lysate/extractB. 30 μg HeLa nuclear lysate/extract15 % SDS-PAGEHistone H3 (tri-Methyl Lys4) antibody (GTX128954) dilution: 1:1000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Histone H3 (tri-Methyl Lys4) antibody detects Histone H3 (tri-Methyl Lys4) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 (tri-Methyl Lys4) protein stained by Histone H3 (tri-Methyl Lys4) antibody (GTX128954) diluted at 1:500. Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-Histone H3K4me3 (trimethyl Lys4) antibody. The precipitated DNA was detected by PCR with primer set targeting to GADPH gene.

Other assay

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Dotblot analysis of anti-Histone H3 (tri-Methyl Lys4) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (tri-Methyl Lys4) antibody (GTX128954) 1:5000 dilution.A: Peptide samples of H3K4 unB: Peptide samples of H3K4me1C: Peptide samples of H3K4me2D: Peptide samples of H3K4me3E: Peptide samples of H3K4ac

Validation comment

Dot