PAB29616

antibody from Abnova Corporation

Targeting: H3C1 H3/A, H3FA, HIST1H3A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29616

Provider

Abnova Corporation

Product name

HIST1H3A (phospho S10) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of human HIST1H3A.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: SK-BR-3 cells, Lane 2: Insulin and EGF treated SK-BR-3 cells with HIST1H3A (phospho S10) polyclonal antibody (Cat# PAB29616) at 1:500-1:1000 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of methanol-fixed Hela cells using HIST1H3A (phospho S10) polyclonal antibody (Cat# PAB29616) at 1:100-1:200 dilution.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human breast cancer tissue by HIST1H3A (phospho S10) polyclonal antibody (Cat# PAB29616) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)