687606

antibody from BioLegend

Targeting: H3C1 H3/A, H3FA, HIST1H3A

Western blot (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

687606

Provider

BioLegend

Proper citation

BioLegend Cat#687606, RRID:AB_2687116

Product name

Go-ChIP-Grade™ Purified anti-Histone H3.1 Phospho (Ser28)

Antibody type

Monoclonal

Reactivity

Human

Host

Rat

Conjugate

Unconjugated

Antibody clone number

5D10D4

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Other assay

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3µg of cross-linked chromatin samples from HeLa cells treated with Nocodazole with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-Histone H3.1 Phospho (Ser28) Antibody (Clone 5D10D4), B) equal amount of Purified Rat IgG2a, κ Isotype Control Antibody, or C) competitor’s ChIP-grade Purified anti-Histone H3.1 Phospho (Ser28) Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human TNF&alpha gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.

Conjugate

Unconjugated