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- Antibody Data
- Antigen structure
- References [42]
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- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00055802-M06 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00055802-M06, RRID:AB_538184
- Product name
- Mouse Monoclonal Dcp1a Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. DCP1A - DCP1 decapping enzyme homolog A (S. cerevisiae)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Reactivation of nonsense-mediated mRNA decay protects against C9orf72 dipeptide-repeat neurotoxicity.
New roles for the de-ubiquitylating enzyme OTUD4 in an RNA-protein network and RNA granules.
FAM98A is localized to stress granules and associates with multiple stress granule-localized proteins.
MOV10 sequesters the RNP of influenza A virus in the cytoplasm and is antagonized by viral NS1 protein.
Intracellular signalling pathways and cytoskeletal functions converge on the psoriasis candidate gene CCHCR1 expressed at P-bodies and centrosomes.
Dazl is a target RNA suppressed by mammalian NANOS2 in sexually differentiating male germ cells.
A Smaug2-Based Translational Repression Complex Determines the Balance between Precursor Maintenance versus Differentiation during Mammalian Neurogenesis.
Polysome arrest restricts miRNA turnover by preventing exosomal export of miRNA in growth-retarded mammalian cells.
Molecular characterization of mouse lens epithelial cell lines and their suitability to study RNA granules and cataract associated genes.
Host cytoplasmic processing bodies assembled by Trypanosoma cruzi during infection exert anti-parasitic activity.
Hepatitis C virus infection inhibits P-body granule formation in human livers.
CCHCR1 interacts with EDC4, suggesting its localization in P-bodies.
C9orf72 nucleotide repeat structures initiate molecular cascades of disease.
Processing bodies accumulate in human cytomegalovirus-infected cells and do not affect viral replication at high multiplicity of infection.
5-Fluorouracil affects assembly of stress granules based on RNA incorporation.
hnRNP L and NF90 interact with hepatitis C virus 5'-terminal untranslated RNA and promote efficient replication.
mRNA decapping enzyme 1a (Dcp1a)-induced translational arrest through protein kinase R (PKR) activation requires the N-terminal enabled vasodilator-stimulated protein homology 1 (EVH1) domain.
The P body protein Dcp1a is hyper-phosphorylated during mitosis.
Screening of small molecules affecting mammalian P-body assembly uncovers links with diverse intracellular processes and organelle physiology.
Pdc1 functions in the assembly of P bodies in Schizosaccharomyces pombe.
Identification and analysis of a novel dimerization domain shared by various members of c-Jun N-terminal kinase (JNK) scaffold proteins.
Zinc-finger antiviral protein mediates retinoic acid inducible gene I-like receptor-independent antiviral response to murine leukemia virus.
Competing and noncompeting activities of miR-122 and the 5' exonuclease Xrn1 in regulation of hepatitis C virus replication.
Identification of DEAD-box RNA helicase 6 (DDX6) as a cellular modulator of vascular endothelial growth factor expression under hypoxia.
Multiple mechanisms repress N-Bak mRNA translation in the healthy and apoptotic neurons.
A monoclonal antibody against p53 cross-reacts with processing bodies.
PKCα binds G3BP2 and regulates stress granule formation following cellular stress.
BUHO: a MATLAB script for the study of stress granules and processing bodies by high-throughput image analysis.
Identification of the P-body component PATL1 as a novel ALG-2-interacting protein by in silico and far-Western screening of proline-rich proteins.
LSm14A is a processing body-associated sensor of viral nucleic acids that initiates cellular antiviral response in the early phase of viral infection.
Critical roles of RNA helicase DDX3 and its interactions with eIF4E/PABP1 in stress granule assembly and stress response.
The NS1 protein of influenza A virus interacts with cellular processing bodies and stress granules through RNA-associated protein 55 (RAP55) during virus infection.
The DEAD-box RNA helicase DDX6 is required for efficient encapsidation of a retroviral genome.
Differential utilization of decapping enzymes in mammalian mRNA decay pathways.
Poliovirus-mediated disruption of cytoplasmic processing bodies.
Smaug1 mRNA-silencing foci respond to NMDA and modulate synapse formation.
c-Jun N-terminal kinase phosphorylates DCP1a to control formation of P bodies.
A novel c-Jun N-terminal kinase (JNK)-binding protein WDR62 is recruited to stress granules and mediates a nonclassical JNK activation.
NANOS2 interacts with the CCR4-NOT deadenylation complex and leads to suppression of specific RNAs.
A large ribonucleoprotein particle induced by cytoplasmic PrP shares striking similarities with the chromatoid body, an RNA granule predicted to function in posttranscriptional gene regulation.
Cytoplasmic compartmentalization of the fetal piRNA pathway in mice.
The dynamics of mammalian P body transport, assembly, and disassembly in vivo.
Xu W, Bao P, Jiang X, Wang H, Qin M, Wang R, Wang T, Yang Y, Lorenzini I, Liao L, Sattler R, Xu J
Brain : a journal of neurology 2019 May 1;142(5):1349-1364
Brain : a journal of neurology 2019 May 1;142(5):1349-1364
New roles for the de-ubiquitylating enzyme OTUD4 in an RNA-protein network and RNA granules.
Das R, Schwintzer L, Vinopal S, Aguado Roca E, Sylvester M, Oprisoreanu AM, Schoch S, Bradke F, Broemer M
Journal of cell science 2019 Jun 17;132(12)
Journal of cell science 2019 Jun 17;132(12)
FAM98A is localized to stress granules and associates with multiple stress granule-localized proteins.
Ozeki K, Sugiyama M, Akter KA, Nishiwaki K, Asano-Inami E, Senga T
Molecular and cellular biochemistry 2019 Jan;451(1-2):107-115
Molecular and cellular biochemistry 2019 Jan;451(1-2):107-115
MOV10 sequesters the RNP of influenza A virus in the cytoplasm and is antagonized by viral NS1 protein.
Li J, Hu S, Xu F, Mei S, Liu X, Yin L, Zhao F, Zhao X, Sun H, Xiong Z, Zhang D, Cen S, Wang J, Liang C, Guo F
The Biochemical journal 2019 Feb 5;476(3):467-481
The Biochemical journal 2019 Feb 5;476(3):467-481
Intracellular signalling pathways and cytoskeletal functions converge on the psoriasis candidate gene CCHCR1 expressed at P-bodies and centrosomes.
Tervaniemi MH, Katayama S, Skoog T, Siitonen HA, Vuola J, Nuutila K, Tammimies K, Suomela S, Kankuri E, Kere J, Elomaa O
BMC genomics 2018 Jun 4;19(1):432
BMC genomics 2018 Jun 4;19(1):432
Dazl is a target RNA suppressed by mammalian NANOS2 in sexually differentiating male germ cells.
Kato Y, Katsuki T, Kokubo H, Masuda A, Saga Y
Nature communications 2016 Apr 13;7:11272
Nature communications 2016 Apr 13;7:11272
A Smaug2-Based Translational Repression Complex Determines the Balance between Precursor Maintenance versus Differentiation during Mammalian Neurogenesis.
Amadei G, Zander MA, Yang G, Dumelie JG, Vessey JP, Lipshitz HD, Smibert CA, Kaplan DR, Miller FD
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Nov 25;35(47):15666-81
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Nov 25;35(47):15666-81
Polysome arrest restricts miRNA turnover by preventing exosomal export of miRNA in growth-retarded mammalian cells.
Ghosh S, Bose M, Ray A, Bhattacharyya SN
Molecular biology of the cell 2015 Mar 15;26(6):1072-83
Molecular biology of the cell 2015 Mar 15;26(6):1072-83
Molecular characterization of mouse lens epithelial cell lines and their suitability to study RNA granules and cataract associated genes.
Terrell AM, Anand D, Smith SF, Dang CA, Waters SM, Pathania M, Beebe DC, Lachke SA
Experimental eye research 2015 Feb;131:42-55
Experimental eye research 2015 Feb;131:42-55
Host cytoplasmic processing bodies assembled by Trypanosoma cruzi during infection exert anti-parasitic activity.
Seto E, Onizuka Y, Nakajima-Shimada J
Parasitology international 2015 Dec;64(6):540-6
Parasitology international 2015 Dec;64(6):540-6
Hepatitis C virus infection inhibits P-body granule formation in human livers.
Pérez-Vilaró G, Fernández-Carrillo C, Mensa L, Miquel R, Sanjuan X, Forns X, Pérez-del-Pulgar S, Díez J
Journal of hepatology 2015 Apr;62(4):785-90
Journal of hepatology 2015 Apr;62(4):785-90
CCHCR1 interacts with EDC4, suggesting its localization in P-bodies.
Ling YH, Wong CC, Li KW, Chan KM, Boukamp P, Liu WK
Experimental cell research 2014 Sep 10;327(1):12-23
Experimental cell research 2014 Sep 10;327(1):12-23
C9orf72 nucleotide repeat structures initiate molecular cascades of disease.
Haeusler AR, Donnelly CJ, Periz G, Simko EA, Shaw PG, Kim MS, Maragakis NJ, Troncoso JC, Pandey A, Sattler R, Rothstein JD, Wang J
Nature 2014 Mar 13;507(7491):195-200
Nature 2014 Mar 13;507(7491):195-200
Processing bodies accumulate in human cytomegalovirus-infected cells and do not affect viral replication at high multiplicity of infection.
Seto E, Inoue T, Nakatani Y, Yamada M, Isomura H
Virology 2014 Jun;458-459:151-61
Virology 2014 Jun;458-459:151-61
5-Fluorouracil affects assembly of stress granules based on RNA incorporation.
Kaehler C, Isensee J, Hucho T, Lehrach H, Krobitsch S
Nucleic acids research 2014 Jun;42(10):6436-47
Nucleic acids research 2014 Jun;42(10):6436-47
hnRNP L and NF90 interact with hepatitis C virus 5'-terminal untranslated RNA and promote efficient replication.
Li Y, Masaki T, Shimakami T, Lemon SM
Journal of virology 2014 Jul;88(13):7199-209
Journal of virology 2014 Jul;88(13):7199-209
mRNA decapping enzyme 1a (Dcp1a)-induced translational arrest through protein kinase R (PKR) activation requires the N-terminal enabled vasodilator-stimulated protein homology 1 (EVH1) domain.
Dougherty JD, Reineke LC, Lloyd RE
The Journal of biological chemistry 2014 Feb 14;289(7):3936-49
The Journal of biological chemistry 2014 Feb 14;289(7):3936-49
The P body protein Dcp1a is hyper-phosphorylated during mitosis.
Aizer A, Kafri P, Kalo A, Shav-Tal Y
PloS one 2013;8(1):e49783
PloS one 2013;8(1):e49783
Screening of small molecules affecting mammalian P-body assembly uncovers links with diverse intracellular processes and organelle physiology.
Martínez JP, Pérez-Vilaró G, Muthukumar Y, Scheller N, Hirsch T, Diestel R, Steinmetz H, Jansen R, Frank R, Sasse F, Meyerhans A, Díez J
RNA biology 2013 Nov;10(11):1661-9
RNA biology 2013 Nov;10(11):1661-9
Pdc1 functions in the assembly of P bodies in Schizosaccharomyces pombe.
Wang CY, Chen WL, Wang SW
Molecular and cellular biology 2013 Mar;33(6):1244-53
Molecular and cellular biology 2013 Mar;33(6):1244-53
Identification and analysis of a novel dimerization domain shared by various members of c-Jun N-terminal kinase (JNK) scaffold proteins.
Cohen-Katsenelson K, Wasserman T, Darlyuk-Saadon I, Rabner A, Glaser F, Aronheim A
The Journal of biological chemistry 2013 Mar 8;288(10):7294-304
The Journal of biological chemistry 2013 Mar 8;288(10):7294-304
Zinc-finger antiviral protein mediates retinoic acid inducible gene I-like receptor-independent antiviral response to murine leukemia virus.
Lee H, Komano J, Saitoh Y, Yamaoka S, Kozaki T, Misawa T, Takahama M, Satoh T, Takeuchi O, Yamamoto N, Matsuura Y, Saitoh T, Akira S
Proceedings of the National Academy of Sciences of the United States of America 2013 Jul 23;110(30):12379-84
Proceedings of the National Academy of Sciences of the United States of America 2013 Jul 23;110(30):12379-84
Competing and noncompeting activities of miR-122 and the 5' exonuclease Xrn1 in regulation of hepatitis C virus replication.
Li Y, Masaki T, Yamane D, McGivern DR, Lemon SM
Proceedings of the National Academy of Sciences of the United States of America 2013 Jan 29;110(5):1881-6
Proceedings of the National Academy of Sciences of the United States of America 2013 Jan 29;110(5):1881-6
Identification of DEAD-box RNA helicase 6 (DDX6) as a cellular modulator of vascular endothelial growth factor expression under hypoxia.
de Vries S, Naarmann-de Vries IS, Urlaub H, Lue H, Bernhagen J, Ostareck DH, Ostareck-Lederer A
The Journal of biological chemistry 2013 Feb 22;288(8):5815-27
The Journal of biological chemistry 2013 Feb 22;288(8):5815-27
Multiple mechanisms repress N-Bak mRNA translation in the healthy and apoptotic neurons.
Jakobson M, Jakobson M, Llano O, Palgi J, Arumäe U
Cell death & disease 2013 Aug 22;4:e777
Cell death & disease 2013 Aug 22;4:e777
A monoclonal antibody against p53 cross-reacts with processing bodies.
Thomas MG, Luchelli L, Pascual M, Gottifredi V, Boccaccio GL
PloS one 2012;7(5):e36447
PloS one 2012;7(5):e36447
PKCα binds G3BP2 and regulates stress granule formation following cellular stress.
Kobayashi T, Winslow S, Sunesson L, Hellman U, Larsson C
PloS one 2012;7(4):e35820
PloS one 2012;7(4):e35820
BUHO: a MATLAB script for the study of stress granules and processing bodies by high-throughput image analysis.
Perez-Pepe M, Slomiansky V, Loschi M, Luchelli L, Neme M, Thomas MG, Boccaccio GL
PloS one 2012;7(12):e51495
PloS one 2012;7(12):e51495
Identification of the P-body component PATL1 as a novel ALG-2-interacting protein by in silico and far-Western screening of proline-rich proteins.
Osugi K, Suzuki H, Nomura T, Ariumi Y, Shibata H, Maki M
Journal of biochemistry 2012 Jun;151(6):657-66
Journal of biochemistry 2012 Jun;151(6):657-66
LSm14A is a processing body-associated sensor of viral nucleic acids that initiates cellular antiviral response in the early phase of viral infection.
Li Y, Chen R, Zhou Q, Xu Z, Li C, Wang S, Mao A, Zhang X, He W, Shu HB
Proceedings of the National Academy of Sciences of the United States of America 2012 Jul 17;109(29):11770-5
Proceedings of the National Academy of Sciences of the United States of America 2012 Jul 17;109(29):11770-5
Critical roles of RNA helicase DDX3 and its interactions with eIF4E/PABP1 in stress granule assembly and stress response.
Shih JW, Wang WT, Tsai TY, Kuo CY, Li HK, Wu Lee YH
The Biochemical journal 2012 Jan 1;441(1):119-29
The Biochemical journal 2012 Jan 1;441(1):119-29
The NS1 protein of influenza A virus interacts with cellular processing bodies and stress granules through RNA-associated protein 55 (RAP55) during virus infection.
Mok BW, Song W, Wang P, Tai H, Chen Y, Zheng M, Wen X, Lau SY, Wu WL, Matsumoto K, Yuen KY, Chen H
Journal of virology 2012 Dec;86(23):12695-707
Journal of virology 2012 Dec;86(23):12695-707
The DEAD-box RNA helicase DDX6 is required for efficient encapsidation of a retroviral genome.
Yu SF, Lujan P, Jackson DL, Emerman M, Linial ML
PLoS pathogens 2011 Oct;7(10):e1002303
PLoS pathogens 2011 Oct;7(10):e1002303
Differential utilization of decapping enzymes in mammalian mRNA decay pathways.
Li Y, Song M, Kiledjian M
RNA (New York, N.Y.) 2011 Mar;17(3):419-28
RNA (New York, N.Y.) 2011 Mar;17(3):419-28
Poliovirus-mediated disruption of cytoplasmic processing bodies.
Dougherty JD, White JP, Lloyd RE
Journal of virology 2011 Jan;85(1):64-75
Journal of virology 2011 Jan;85(1):64-75
Smaug1 mRNA-silencing foci respond to NMDA and modulate synapse formation.
Baez MV, Luchelli L, Maschi D, Habif M, Pascual M, Thomas MG, Boccaccio GL
The Journal of cell biology 2011 Dec 26;195(7):1141-57
The Journal of cell biology 2011 Dec 26;195(7):1141-57
c-Jun N-terminal kinase phosphorylates DCP1a to control formation of P bodies.
Rzeczkowski K, Beuerlein K, Müller H, Dittrich-Breiholz O, Schneider H, Kettner-Buhrow D, Holtmann H, Kracht M
The Journal of cell biology 2011 Aug 22;194(4):581-96
The Journal of cell biology 2011 Aug 22;194(4):581-96
A novel c-Jun N-terminal kinase (JNK)-binding protein WDR62 is recruited to stress granules and mediates a nonclassical JNK activation.
Wasserman T, Katsenelson K, Daniliuc S, Hasin T, Choder M, Aronheim A
Molecular biology of the cell 2010 Jan 1;21(1):117-30
Molecular biology of the cell 2010 Jan 1;21(1):117-30
NANOS2 interacts with the CCR4-NOT deadenylation complex and leads to suppression of specific RNAs.
Suzuki A, Igarashi K, Aisaki K, Kanno J, Saga Y
Proceedings of the National Academy of Sciences of the United States of America 2010 Feb 23;107(8):3594-9
Proceedings of the National Academy of Sciences of the United States of America 2010 Feb 23;107(8):3594-9
A large ribonucleoprotein particle induced by cytoplasmic PrP shares striking similarities with the chromatoid body, an RNA granule predicted to function in posttranscriptional gene regulation.
Beaudoin S, Vanderperre B, Grenier C, Tremblay I, Leduc F, Roucou X
Biochimica et biophysica acta 2009 Feb;1793(2):335-45
Biochimica et biophysica acta 2009 Feb;1793(2):335-45
Cytoplasmic compartmentalization of the fetal piRNA pathway in mice.
Aravin AA, van der Heijden GW, Castañeda J, Vagin VV, Hannon GJ, Bortvin A
PLoS genetics 2009 Dec;5(12):e1000764
PLoS genetics 2009 Dec;5(12):e1000764
The dynamics of mammalian P body transport, assembly, and disassembly in vivo.
Aizer A, Brody Y, Ler LW, Sonenberg N, Singer RH, Shav-Tal Y
Molecular biology of the cell 2008 Oct;19(10):4154-66
Molecular biology of the cell 2008 Oct;19(10):4154-66
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Dcp1a Antibody (3G4) [H00055802-M06] - Western Blot analysis of DCP1A expression in IMR-32 ( Cat # L008V1 ).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Dcp1a Antibody (3G4) [H00055802-M06] - Detection limit for recombinant GST tagged DCP1A is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Dcp1a Antibody (3G4) [H00055802-M06] - Analysis of monoclonal antibody to DCP1A on HeLa cell. Antibody concentration 10 ug/ml
