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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [1]
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- Product number
- MAB7500 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Lck Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Lck when phosphorylated at Y394. Due to high sequence homology, this antibody may cross-react with phosphorylated members of the Src family.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Nuclear pore complex-mediated modulation of TCR signaling is required for naïve CD4+ T cell homeostasis.
Characterization of the effects of immunomodulatory drug fingolimod (FTY720) on human T cell receptor signaling pathways.
TAOK3 Regulates Canonical TCR Signaling by Preventing Early SHP-1-Mediated Inactivation of LCK.
Hepatitis C virus infection inhibits a Src-kinase regulatory phosphatase and reduces T cell activation in vivo.
T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases.
Borlido J, Sakuma S, Raices M, Carrette F, Tinoco R, Bradley LM, D'Angelo MA
Nature immunology 2018 Jun;19(6):594-605
Nature immunology 2018 Jun;19(6):594-605
Characterization of the effects of immunomodulatory drug fingolimod (FTY720) on human T cell receptor signaling pathways.
Baer A, Colon-Moran W, Bhattarai N
Scientific reports 2018 Jul 19;8(1):10910
Scientific reports 2018 Jul 19;8(1):10910
TAOK3 Regulates Canonical TCR Signaling by Preventing Early SHP-1-Mediated Inactivation of LCK.
Ormonde JVS, Li Z, Stegen C, Madrenas J
Journal of immunology (Baltimore, Md. : 1950) 2018 Dec 1;201(11):3431-3442
Journal of immunology (Baltimore, Md. : 1950) 2018 Dec 1;201(11):3431-3442
Hepatitis C virus infection inhibits a Src-kinase regulatory phosphatase and reduces T cell activation in vivo.
Bhattarai N, McLinden JH, Xiang J, Mathahs MM, Schmidt WN, Kaufman TM, Stapleton JT
PLoS pathogens 2017 Feb;13(2):e1006232
PLoS pathogens 2017 Feb;13(2):e1006232
T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases.
Clayton KL, Haaland MS, Douglas-Vail MB, Mujib S, Chew GM, Ndhlovu LC, Ostrowski MA
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 15;192(2):782-91
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 15;192(2):782-91
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Phospho-Lck (Y394) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Phospho-Lck (Y394) Monoclonal Antibody (Catalog # MAB7500) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-Lck (Y394) at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
