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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB0081 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB0081, RRID:AB_1018954
- Product name
- IKBKE monoclonal antibody, clone 72B587
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein corresponding to amino acids 175-188 of human IKBKE.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 μg
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Interferon regulatory factor 3-dependent responses to lipopolysaccharide are selectively blunted in cord blood cells.
Involvement of the IkappaB kinase (IKK)-related kinases tank-binding kinase 1/IKKi and cullin-based ubiquitin ligases in IFN regulatory factor-3 degradation.
Association of the adaptor TANK with the I kappa B kinase (IKK) regulator NEMO connects IKK complexes with IKK epsilon and TBK1 kinases.
Aksoy E, Albarani V, Nguyen M, Laes JF, Ruelle JL, De Wit D, Willems F, Goldman M, Goriely S
Blood 2007 Apr 1;109(7):2887-93
Blood 2007 Apr 1;109(7):2887-93
Involvement of the IkappaB kinase (IKK)-related kinases tank-binding kinase 1/IKKi and cullin-based ubiquitin ligases in IFN regulatory factor-3 degradation.
Bibeau-Poirier A, Gravel SP, Clément JF, Rolland S, Rodier G, Coulombe P, Hiscott J, Grandvaux N, Meloche S, Servant MJ
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5059-67
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5059-67
Association of the adaptor TANK with the I kappa B kinase (IKK) regulator NEMO connects IKK complexes with IKK epsilon and TBK1 kinases.
Chariot A, Leonardi A, Muller J, Bonif M, Brown K, Siebenlist U
The Journal of biological chemistry 2002 Oct 4;277(40):37029-36
The Journal of biological chemistry 2002 Oct 4;277(40):37029-36
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of IKBKE. Using IKBKE monoclonal antibody, clone 72B587 (Cat # MAB0081) at 2 ug/mL on 15 ugs of Jurkat cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of IKBKE in 106 NIH/3T3 cells using 0.1 ug of IKBKE monoclonal antibody, clone 72B587 (Cat # MAB0081). Shaded histogram represents cells without antibody; Green represents isotype control; red represents anti-IKKg antibody.
- Validation comment
- Flow Cytometry
