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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-32807 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IKK epsilon Recombinant Rabbit Monoclonal Antibody (JU06-72)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JU06-72
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IKK epsilon in different lysates using a Monoclonal antibody (Product #MA5-32807) at a dilution of 1:500. Positive control: Lane 1: Hela, Lane 2: Raji, Lane 3: MCF-7, Lane 4: Jurkat.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-IKK epsilon Rabbit Monoclonal Antibody (JU06-72) (Product # MA5-32807) and an 80 kDa band corresponding to IKK epsilon was observed in all cell lines and tissues tested. Expression of IKK epsilon was higher in MDA-MB-231 as compared to MCF 10A [10.1016/j.cell.2007.03.052]. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MCF 10A (Lane 2), T98G (Lane 3), SH-SY5Y (Lane 4) and Mouse Thymus (lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of IKK epsilon in Hela cells using a IKK epsilon Monoclonal antibody (Product # MA5-32807) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of IKK epsilon in JAR cells using a IKK epsilon Monoclonal antibody (Product # MA5-32807) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of IKK epsilon in SK-Br-3 cells using a IKK epsilon Monoclonal antibody (Product # MA5-32807) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IKK epsilon of paraffin-embedded Human kidney tissue using a IKK-epsilon Monoclonal antibody (Product #MA5-32807). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of IKK epsilon in Jurkat cells using a IKK epsilon Monoclonal Antibody (Product # MA5-32807) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
