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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- GTX12142 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX12142, RRID:AB_381370
- Product name
- IKBKE antibody [72B587]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Storage
- Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of IKKi at 2 μg/ml on 15 μgs of Jurkat cell lysate
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IKK iota/IKK epsilon (80 kDa) detection by Western blot. The analysis of 10 g of total cell extract from Daudi cells with the anti-IKK iota/IKK epsilon at 0.5 g/ml (lane 1) and 3 g/ml (lane 2) dilution
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of A) human Daudi, B) HeLa, and C) mouse NIH3T3 lysate probed with IKKbeta antibody at 2 μg/ml.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human gall bladder probed with IKKbeta antibody at 5 μg/ml. IMGENEXs human tissue TMA was used for this test.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Intracellular staining of HEK293 cells with 0.5 μg of IKKbeta antibody (red) and 0.5 μg of isotype control antibody (green). IMGENEX's intracellular flow kit was used for this test, and an anti-mouse IgG1 PE conjμgated secondary.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of IKK epsilon in 10^6 NIH 3T3°Cells using 0.1 μg. Shaded histogram represents cells without antibody; green represents isotype control; red represents IKK epsilon antibody. A goat anti-mouse IgG: FITC secondary antibody was used for this test.
