NBP1-76800

antibody from Novus Biologicals

Targeting: IKBKE IKK-i, IKKE, KIAA0151

ELISA

Immunocytochemistry

Antibody data

Submit

Validation data

Product number: NBP1-76800 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NBP1-76800, RRID:AB_11020664
Product name: Rabbit Polyclonal IKK epsilon/IKBKE Antibody
Antibody type: Polyclonal
Description: Peptide affinity purified. It has no cross response to IKKa, IKKb, or IKKy.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 0.1 mg
Concentration: 1 mg/ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

IKBKE protein structure - NBP1-76800 shown in red.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: IKK epsilon/IKBKE Antibody [NBP1-76800] - Analysis of IKK e in Jurkat whole cell lysate using NBP1-76800 at 1 ug/ml.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: IKK epsilon/IKBKE Antibody [NBP1-76800] - Western Blot Validation in Jurkat Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: IKK epsilon/IKBKE NBP1-76800 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: IKK epsilon/IKBKE Antibody [NBP1-76800] - Western Blot Validation in Mouse Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: IKK epsilon/IKBKE NBP1-76800 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: IKK epsilon/IKBKE Antibody [NBP1-76800] - Western Blot Validation in Human and Mouse Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: IKK epsilon/IKBKE NBP1-76800 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: IKK epsilon/IKBKE Antibody [NBP1-76800] - Induced Expression Validation in Mouse Cell Line. Loading: 15 ug of RAW 264.7 cell lysates per lane. Antibodies: IKK epsilon/IKBKE NBP1-76800 (1 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Cells were treated with LPS (0.3 ug/ml) for 3 hrs (lane 2) and 6 hrs (lane 3) or with control (lane 1).