Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX103216 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX103216, RRID:AB_10732171
- Product name
- alpha Actinin 3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7.5% SDS PAGE GTX103216 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: PC-12 7.5% SDS PAGE GTX103216 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- alpha Actinin 3 antibody detects alpha Actinin 3 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 3 antibody (GTX103216) diluted by 1:10000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE GTX103216 diluted at 1:10000
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of alpha Actinin 3 protein from HeLa whole cell extracts using 5 £gg of alpha Actinin 3 antibody (GTX103216).Western blot analysis was performed using alpha Actinin 3 antibody (GTX103216).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
