PA5-36233

antibody from Invitrogen Antibodies

Targeting: ACTN3

Antibody data

Submit

Validation data

Product number: PA5-36233 - Provider product page
Provider: Invitrogen Antibodies
Product name: alpha Actinin 3 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This antibody detects endogenous protein at a molecular weight of 103 kDa. It does not cross-react with other alpha-actinin isoforms.
Concentration: 1 mg/mL

ACTN3 protein structure - PA5-36233 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of alpha Actinin 3 in Lane 1: MCF-7 whole cell lysate (40 µg), Lane 2: HeLa whole cell lysate (40 µg), Lane 3: BV2 whole cell lysate (40 µg), Lane 4: C6 whole cell lysate (40 µg). Samples were incubated with alpha Actinin 3 polyclonal antibody (Product # PA5-36233) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of alpha Actinin 3 in Lane 1: HeLa cell lysate, Lane 2: mouse muscle tissue lysate, Lane 3: rat muscle tissue lysate. Samples were incubated with alpha Actinin 3 polyclonal antibody (Product # PA5-36233) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of alpha Actinin 3 in Lane 1: MCF-7 whole cell lysate (40 µg), Lane 2: HeLa whole cell lysate (40 µg), Lane 3: BV2 whole cell lysate (40 µg), Lane 4: C6 whole cell lysate (40 µg). Samples were incubated with alpha Actinin 3 polyclonal antibody (Product # PA5-36233) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of alpha Actinin 3 in Lane 1: HeLa cell lysate, Lane 2: mouse muscle tissue lysate, Lane 3: rat muscle tissue lysate. Samples were incubated with alpha Actinin 3 polyclonal antibody (Product # PA5-36233) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of alpha Actinin 3 using alpha Actinin 3 polyclonal antibody (Product # PA5-36233) at a dilution of 1:500. Lane 1: Hela cell lysate, Lane 2: Mouse muscle tissue lysate, Lane 3: Rat muscel tissue lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of alpha Actinin 3 in paraffin-embedded human tonsil carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with alpha Actinin 3 polyclonal antibody (Product # PA5-36233) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of alpha Actinin 3 in paraffin-embedded human lung carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with alpha Actinin 3 polyclonal antibody (Product # PA5-36233) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of alpha Actinin 3 in paraffin-embedded human colon carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with alpha Actinin 3 polyclonal antibody (Product # PA5-36233) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of alpha Actinin 3 in paraffin-embedded human kidney carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with alpha Actinin 3 polyclonal antibody (Product # PA5-36233) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.