Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-38605 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-alpha Actinin 2/3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of alpha Actinin 2 + alpha Actinin 3 in extracts from HeLa cells using an Alpha Actinin 2 + alpha Actinin 3 polyclonal antibody (Product # PA5-38605).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-alpha Actinin 2/3 Polyclonal Antibody (Product # PA5-38605) and a 110 kDa band corresponding to alpha Actinin 2/3 was high in Mouse and Rat Skeletal Muscle which are reported as high expressing models when compared to other tissues. Tissue extracts (30 µg lysate) of Mouse Skeletal Muscle (Lane 1), Rat Skeletal Muscle (Lane 2), Rat Heart (Lane 3), Mouse Heart (Lane 4), Rat Lung (Lane 5), Mouse Lung (Lane 6) and Mouse Stomach (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution), using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of alpha Actinin 2 + alpha Actinin 3 in HeLa cells using an Alpha Actinin 2 + alpha Actinin 3 polyclonal antibody (Product # PA5-38605).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of alpha Actinin 2 + alpha Actinin 3 in HeLa cells using an Alpha Actinin 2 + alpha Actinin 3 polyclonal antibody (Product # PA5-38605).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of alpha Actinin 2 + alpha Actinin 3 in paraffin-embedded human skeletal muscle tissue using an Alpha Actinin 2 + alpha Actinin 3 polyclonal antibody (Product # PA5-38605).