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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- M02693 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-ACTN3 Antibody Picoband™ (monoclonal, 9B5)
- Antibody type
- Monoclonal
- Description
- Mouse IgG monoclonal antibody for ACTN3 detection. Tested with WB, IHC-P in Mouse;Rat.
- Reactivity
- Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 9B5
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of ACTN3 using anti-ACTN3 antibody (M02693). ACTN3 was detected in paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-ACTN3 Antibody (M02693) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
- Additional image
