AF802
antibody from R&D Systems
Targeting: CCL16
CKb12, HCC-4, LCC-1, LEC, LMC, Mtn-1, NCC-4, SCYA16, SCYL4
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF802 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL16/HCC-4 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CCL16/HCC-4 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O15467
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium.
Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis.
Rump L, Mattey DL, Kehoe O, Middleton J
Cytokine 2017 Sep;97:133-140
Cytokine 2017 Sep;97:133-140
Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis.
Haringman JJ, Smeets TJ, Reinders-Blankert P, Tak PP
Annals of the rheumatic diseases 2006 Mar;65(3):294-300
Annals of the rheumatic diseases 2006 Mar;65(3):294-300
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL16/HCC-4 and Neutralization by Human CCL16/HCC-4 Antibody. Recombinant Human CCL16/HCC-4 (Catalog # 802-HC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL16/HCC-4 (750 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL16/HCC-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF802). The ND50 is typically 4-16 µg/mL.