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LearnMAB328
antibody from R&D Systems
Targeting: CCL16
CKb12, HCC-4, LCC-1, LEC, LMC, Mtn-1, NCC-4, SCYA16, SCYL4
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB328 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL16/HCC-4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human CCL16/HCC-4 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O15467- Isotype
- IgG
- Antibody clone number
- 70218
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references CCL16/LEC powerfully triggers effector and antigen-presenting functions of macrophages and enhances T cell cytotoxicity.
Cappello P, Caorsi C, Bosticardo M, De Angelis S, Novelli F, Forni G, Giovarelli M
Journal of leukocyte biology 2004 Jan;75(1):135-42
Journal of leukocyte biology 2004 Jan;75(1):135-42
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL16/HCC-4 and Neutral-ization by Human CCL16/ HCC-4 Antibody. Recombinant Human CCL16/ HCC-4 (Catalog # 802-HC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL16/ HCC-4 (0.75 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL16/ HCC-4 Monoclonal Antibody (Catalog # MAB328). The ND50 is typically 5-20 µg/mL.
