H00004101-M01

antibody from Abnova Corporation

Targeting: MAGEA2 CT1.2, MAGE2, MAGEA2A

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004101-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004101-M01, RRID:AB_425535

Product name

MAGEA2 monoclonal antibody (M01), clone 1H4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant MAGEA2.

Antigen sequence

MPLEQRSQHCKPEEGLEARGEALGLVGAQAPATEE
QQTASSSSTLVEVTLGEVPAADSPSPPHSPQGASS
FSTTINYTLWRQSDEGSSNQEEEGPRMFPDLESEF
QAAISRKMVELVHFLLLKYQAREPVTKAEMLESVL
RNCQDFFPVIFSKASEYLQLVFGIEVVEVVPISHL
YILVTCLGLSYDGLLGDNQVMPKTGLLIIVLAIIA
IEGDCAPEEKIWEELSMLEVFEGREDSVFAHPRKL
LMQDLVQENYLEYRQVPGSDPACYEFLWGPRALIE
TSYVKVLHHTLKIGGEPHISYPPLHERALREGEE


Isotype

IgG

Antibody clone number

1H4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

MAGEA2 monoclonal antibody (M01), clone 1H4 Western Blot analysis of MAGEA2 expression in Jurkat ( Cat # L017V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MAGEA2 expression in transfected 293T cell line by MAGEA2 monoclonal antibody (M01), clone 1H4.Lane 1: MAGEA2 transfected lysate(35 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged MAGEA2 is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol