828004

antibody from BioLegend

Targeting: H4C1 H4FA, HIST1H4A

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

828004

Provider

BioLegend

Proper citation

BioLegend Cat#828004, RRID:AB_2632843

Product name

Go-ChIP-Grade™ Purified anti-Histone H4 Monomethyl (Lys20)

Antibody type

Monoclonal

Reactivity

Human, Rat

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

5E10-D8

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Other assay

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from Jurkat cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-Histone H4 Monomethyl (Lys20) (clone 5E10-D8), B) equal amount of Purified Mouse IgG1 Isotype Control Antibody (clone MG1-45), or C) competitor’s ChIP-grade Purified anti-Histone H4 Monomethyl (Lys20) Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human GAPDH gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.

Conjugate

Unconjugated