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LearnAF363
antibody from Novus Biologicals
Targeting: CCL15
HCC-2, HMRP-2B, Lkn-1, MIP-1d, MIP-5, NCC-3, SCYA15, SCYL3
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF363 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal CCL15/MIP-1 delta Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CCL15/MIP-1 delta in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) MPIF-1 is observed and less than 1% cross-reactivity with rhMIP-1 alpha is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Macrophage inflammatory protein-1 delta: a novel osteoclast stimulating factor secreted by renal cell carcinoma bone metastasis.
Kominsky SL, Abdelmagid SM, Doucet M, Brady K, Weber KL
Cancer research 2008 Mar 1;68(5):1261-6
Cancer research 2008 Mar 1;68(5):1261-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL15/MIP-1 delta and Neutralization by Human CCL15/MIP-1 delta Antibody. Recombinant Human CCL15/MIP-1 delta (Catalog # 363-MG) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL15/MIP-1 delta (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL15/MIP-1 delta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF363). The ND50 is typically 1-5 µg/mL.
