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LearnNB100-77417
antibody from Novus Biologicals
Targeting: PTPRC
CD45, GP180, LCA, T200
Western blot Immunocytochemistry Immunoprecipitation
Immunohistochemistry Flow cytometry Functional assayAntibody data
- Antibody Data
- Antigen structure
- References [24]
- Comments [0]
- Validations
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- NB100-77417 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-77417, RRID:AB_1083776
- Product name
- Rat Monoclonal CD45 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects mouse CD45. Recognizes all CD45 isoforms as well as the known mouse CD45 alloantigens (CD45-1/Ly 5a and CD45-2/Ly 5b)
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.5 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references 18F-FAC PET Visualizes Brain-Infiltrating Leukocytes in a Mouse Model of Multiple Sclerosis.
Preventive effects of folic acid on Zika virus-associated poor pregnancy outcomes in immunocompromised mice.
Electron paramagnetic resonance spectroscopy reveals alterations in the redox state of endogenous copper and iron complexes in photodynamic stress-induced ischemic mouse liver.
Enterovirus 71 targets the cardiopulmonary system in a robust oral infection mouse model.
The milk thistle (Silybum marianum) compound Silibinin stimulates leukopoiesis from mouse embryonic stem cells.
Parabiosis and single-cell RNA sequencing reveal a limited contribution of monocytes to myofibroblasts in kidney fibrosis.
Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells.
Bone Marrow-Derived Cell Recruitment to the Neurosensory Retina and Retinal Pigment Epithelial Cell Layer Following Subthreshold Retinal Phototherapy.
Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration.
SEC23B is required for pancreatic acinar cell function in adult mice.
Leukocyte opioid receptors mediate analgesia via Ca(2+)-regulated release of opioid peptides.
Synthesis and cytotoxic activity of novel A-ring cleaved ursolic acid derivatives in human non-small cell lung cancer cells.
Chronic liver inflammation and hepatocellular carcinogenesis are independent of S100A9.
Overexpression of branched O-linked oligosaccharides on T cell surface glycoproteins impairs humoral immune responses in transgenic mice.
Overexpression of HOX11 leads to the immortalization of embryonic precursors with both primitive and definitive hematopoietic potential.
Involvement of CD45 in adhesion and suppression of apoptosis of mouse malignant T-lymphoma cells.
Distinct involvement of CD45 in antigen receptor signalling in CD4+ and CD8+ primary T cells.
Selective radiation of hematolymphoid tissue delivered by anti-CD45 antibody.
Natural autoantibodies to thymocytes: origin, VH genes, fine specificities, and the role of Thy-1 glycoprotein.
Induction of immune tolerance by administration of monoclonal antibody to L3T4.
Comparison of response to stem cell differentiation signals between normal and autoimmune mouse strains.
Expression of antigens of the 'T200' family of glycoproteins on hemopoietic stem cells: evidence that thymocyte cell lineage antigens are represented on 'T200'.
Treatment of autoimmune MRL/Ipr mice with monoclonal antibody to Thy-1.2: a single injection has sustained effects on lymphoproliferation and renal disease.
Surface antigens on mouse natural killer cells: use of monoclonal antibodies to inhibit or to enrich cytotoxic activity.
Chen BY, Ghezzi C, Villegas B, Quon A, Radu CG, Witte ON, Clark PM
Journal of nuclear medicine : official publication, Society of Nuclear Medicine 2020 May;61(5):757-763
Journal of nuclear medicine : official publication, Society of Nuclear Medicine 2020 May;61(5):757-763
Preventive effects of folic acid on Zika virus-associated poor pregnancy outcomes in immunocompromised mice.
Simanjuntak Y, Ko HY, Lee YL, Yu GY, Lin YL
PLoS pathogens 2020 May;16(5):e1008521
PLoS pathogens 2020 May;16(5):e1008521
Electron paramagnetic resonance spectroscopy reveals alterations in the redox state of endogenous copper and iron complexes in photodynamic stress-induced ischemic mouse liver.
Jakubowska MA, Pyka J, Michalczyk-Wetula D, Baczyński K, Cieśla M, Susz A, Ferdek PE, Płonka BK, Fiedor L, Płonka PM
Redox biology 2020 Jul;34:101566
Redox biology 2020 Jul;34:101566
Enterovirus 71 targets the cardiopulmonary system in a robust oral infection mouse model.
Chang CS, Liao CC, Liou AT, Chang YS, Chang YT, Tzeng BH, Chen CC, Shih C
Scientific reports 2019 Jul 31;9(1):11108
Scientific reports 2019 Jul 31;9(1):11108
The milk thistle (Silybum marianum) compound Silibinin stimulates leukopoiesis from mouse embryonic stem cells.
Sharifpanah F, Ali EH, Wartenberg M, Sauer H
Phytotherapy research : PTR 2019 Feb;33(2):452-460
Phytotherapy research : PTR 2019 Feb;33(2):452-460
Parabiosis and single-cell RNA sequencing reveal a limited contribution of monocytes to myofibroblasts in kidney fibrosis.
Kramann R, Machado F, Wu H, Kusaba T, Hoeft K, Schneider RK, Humphreys BD
JCI insight 2018 May 3;3(9)
JCI insight 2018 May 3;3(9)
Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells.
Samanta D, Park Y, Ni X, Li H, Zahnow CA, Gabrielson E, Pan F, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2018 Feb 6;115(6):E1239-E1248
Proceedings of the National Academy of Sciences of the United States of America 2018 Feb 6;115(6):E1239-E1248
Bone Marrow-Derived Cell Recruitment to the Neurosensory Retina and Retinal Pigment Epithelial Cell Layer Following Subthreshold Retinal Phototherapy.
Caballero S, Kent DL, Sengupta N, Li Calzi S, Shaw L, Beli E, Moldovan L, Dominguez JM 2nd, Moorthy RS, Grant MB
Investigative ophthalmology & visual science 2017 Oct 1;58(12):5164-5176
Investigative ophthalmology & visual science 2017 Oct 1;58(12):5164-5176
Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration.
Ambrosi TH, Scialdone A, Graja A, Gohlke S, Jank AM, Bocian C, Woelk L, Fan H, Logan DW, Schürmann A, Saraiva LR, Schulz TJ
Cell stem cell 2017 Jun 1;20(6):771-784.e6
Cell stem cell 2017 Jun 1;20(6):771-784.e6
SEC23B is required for pancreatic acinar cell function in adult mice.
Khoriaty R, Vogel N, Hoenerhoff MJ, Sans MD, Zhu G, Everett L, Nelson B, Durairaj H, McKnight B, Zhang B, Ernst SA, Ginsburg D, Williams JA
Molecular biology of the cell 2017 Jul 15;28(15):2146-2154
Molecular biology of the cell 2017 Jul 15;28(15):2146-2154
Leukocyte opioid receptors mediate analgesia via Ca(2+)-regulated release of opioid peptides.
Celik MÖ, Labuz D, Henning K, Busch-Dienstfertig M, Gaveriaux-Ruff C, Kieffer BL, Zimmer A, Machelska H
Brain, behavior, and immunity 2016 Oct;57:227-242
Brain, behavior, and immunity 2016 Oct;57:227-242
Synthesis and cytotoxic activity of novel A-ring cleaved ursolic acid derivatives in human non-small cell lung cancer cells.
Mendes VIS, Bartholomeusz GA, Ayres M, Gandhi V, Salvador JAR
European journal of medicinal chemistry 2016 Nov 10;123:317-331
European journal of medicinal chemistry 2016 Nov 10;123:317-331
Chronic liver inflammation and hepatocellular carcinogenesis are independent of S100A9.
De Ponti A, Wiechert L, Stojanovic A, Longerich T, Marhenke S, Hogg N, Vogel A, Cerwenka A, Schirmacher P, Hess J, Angel P
International journal of cancer 2015 May 15;136(10):2458-63
International journal of cancer 2015 May 15;136(10):2458-63
Overexpression of branched O-linked oligosaccharides on T cell surface glycoproteins impairs humoral immune responses in transgenic mice.
Tsuboi S, Fukuda M
The Journal of biological chemistry 1998 Nov 13;273(46):30680-7
The Journal of biological chemistry 1998 Nov 13;273(46):30680-7
Overexpression of HOX11 leads to the immortalization of embryonic precursors with both primitive and definitive hematopoietic potential.
Keller G, Wall C, Fong AZ, Hawley TS, Hawley RG
Blood 1998 Aug 1;92(3):877-87
Blood 1998 Aug 1;92(3):877-87
Involvement of CD45 in adhesion and suppression of apoptosis of mouse malignant T-lymphoma cells.
Hanaoka K, Fujita N, Lee SH, Seimiya H, Naito M, Tsuruo T
Cancer research 1995 May 15;55(10):2186-90
Cancer research 1995 May 15;55(10):2186-90
Distinct involvement of CD45 in antigen receptor signalling in CD4+ and CD8+ primary T cells.
Maroun CR, Julius M
European journal of immunology 1994 Apr;24(4):967-73
European journal of immunology 1994 Apr;24(4):967-73
Selective radiation of hematolymphoid tissue delivered by anti-CD45 antibody.
Matthews DC, Badger CC, Fisher DR, Hui TE, Nourigat C, Appelbaum FR, Martin PJ, Bernstein ID
Cancer research 1992 Mar 1;52(5):1228-34
Cancer research 1992 Mar 1;52(5):1228-34
Natural autoantibodies to thymocytes: origin, VH genes, fine specificities, and the role of Thy-1 glycoprotein.
Hayakawa K, Carmack CE, Hyman R, Hardy RR
The Journal of experimental medicine 1990 Sep 1;172(3):869-78
The Journal of experimental medicine 1990 Sep 1;172(3):869-78
Induction of immune tolerance by administration of monoclonal antibody to L3T4.
Gutstein NL, Seaman WE, Scott JH, Wofsy D
Journal of immunology (Baltimore, Md. : 1950) 1986 Aug 15;137(4):1127-32
Journal of immunology (Baltimore, Md. : 1950) 1986 Aug 15;137(4):1127-32
Comparison of response to stem cell differentiation signals between normal and autoimmune mouse strains.
Raveche ES, Chused TM, Steinberg AD, Laskin CA, Edison LJ, Tjio JH
Journal of immunology (Baltimore, Md. : 1950) 1985 Feb;134(2):865-71
Journal of immunology (Baltimore, Md. : 1950) 1985 Feb;134(2):865-71
Expression of antigens of the 'T200' family of glycoproteins on hemopoietic stem cells: evidence that thymocyte cell lineage antigens are represented on 'T200'.
Ralph SJ, Berridge MV
Journal of immunology (Baltimore, Md. : 1950) 1984 May;132(5):2510-4
Journal of immunology (Baltimore, Md. : 1950) 1984 May;132(5):2510-4
Treatment of autoimmune MRL/Ipr mice with monoclonal antibody to Thy-1.2: a single injection has sustained effects on lymphoproliferation and renal disease.
Seaman WE, Wofsy D, Greenspan JS, Ledbetter JA
Journal of immunology (Baltimore, Md. : 1950) 1983 Apr;130(4):1713-8
Journal of immunology (Baltimore, Md. : 1950) 1983 Apr;130(4):1713-8
Surface antigens on mouse natural killer cells: use of monoclonal antibodies to inhibit or to enrich cytotoxic activity.
Seaman WE, Talal N, Herzenberg LA, Herzenberg LA, Ledbetter JA
Journal of immunology (Baltimore, Md. : 1950) 1981 Sep;127(3):982-6
Journal of immunology (Baltimore, Md. : 1950) 1981 Sep;127(3):982-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Analysis of a FFPE section of mouse spleen using rat monoclonal CD45 antibody (clone 30-F11) at 1:50 dilution. The antibody generated an expected membrane staining in the red and white pulp spleenocytes of the tested spleen section.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Analysis of FFPE section of mouse spleen using rat monoclonal CD45 antibody (clone 30-F11) at 1:50 dilution. The antibody generated an expected membrane staining in the red and white pulp spleenocytes of the tested spleen section.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of a FFPE embedded tissue section of mouse liver using 1:100 dilution of anti-CD45 antibody. The signal was developed using AEC reagent based method with hematoxylin counterstaining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: CD45 Antibody (30-F11) [NB100-77417] - Analysis of a mouse spleen cryosection using CD45 antibody clone 30-F11 at 1:50 dilution. The signal was detected using goat anti-rat Alexa fluor 488 secondary with DAPI counterstaining. Image submitted via a verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of CD45 (30F11) in freshly isolated mouse (Balb C) splenocytes (1x10^6) were stained with CD45 antibody (NB100-77417) at 1:1000 dilution and detected with FITC conjugated donkey anti-rat IgG secondary (orange). Shown with rat IgG isotype control (blue).
