Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 86-0452-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD45R (B220) Monoclonal Antibody (RA3-6B2), eVolve 655, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen. Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This RA3-6B2 antibody has been pre-titrated and tested by flow cytometric analysis of mouse splenocytes. This can be used at 5 µL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For best results when using antibodies conjugated to eVolve, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip. Laser/Filter Recommendation: eVolve 655 can be optimally excited with laser lines from 325-405 nm, and emits near 655 nm. We recommend using the violet (405 nm) laser line for excitation and a 630LP with a 660/40 bandpass filter, or equivalent, for detection. Fixation Recommendation: Antibodies conjugated to eVolve are compatible with eBioscience's fixation and permeabilization buffers. Excitation: 350-635 nm; Emission: 657 nm; Laser: Ultraviolet Laser, Violet Laser Storage and handling: For best results when using antibodies conjugated to eVolve, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- RA3-6B2
- Vial size
- 100 Tests
- Concentration
- 5 µl/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references ATF3 Sustains IL-22-Induced STAT3 Phosphorylation to Maintain Mucosal Immunity Through Inhibiting Phosphatases.
Adrenergic nerve degeneration in bone marrow drives aging of the hematopoietic stem cell niche.
Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.
A role for brain-derived neurotrophic factor in B cell development.
Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues.
Glal D, Sudhakar JN, Lu HH, Liu MC, Chiang HY, Liu YC, Cheng CF, Shui JW
Frontiers in immunology 2018;9:2522
Frontiers in immunology 2018;9:2522
Adrenergic nerve degeneration in bone marrow drives aging of the hematopoietic stem cell niche.
Maryanovich M, Zahalka AH, Pierce H, Pinho S, Nakahara F, Asada N, Wei Q, Wang X, Ciero P, Xu J, Leftin A, Frenette PS
Nature medicine 2018 Jun;24(6):782-791
Nature medicine 2018 Jun;24(6):782-791
Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.
Wang Y, Xiao M, Tao C, Chen J, Wang Z, Yang J, Chen Z, Zou Z, Liu A, Cai D, Jiang Y, Ding C, Li M, Bai X
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2018 Apr;33(4):732-742
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2018 Apr;33(4):732-742
A role for brain-derived neurotrophic factor in B cell development.
Schuhmann B, Dietrich A, Sel S, Hahn C, Klingenspor M, Lommatzsch M, Gudermann T, Braun A, Renz H, Nockher WA
Journal of neuroimmunology 2005 Jun;163(1-2):15-23
Journal of neuroimmunology 2005 Jun;163(1-2):15-23
Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues.
Monteith CE, Chelack BJ, Davis WC, Haines DM
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire 1996 Jul;60(3):193-8
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire 1996 Jul;60(3):193-8
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of mouse splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and Anti-Human/Mouse CD45R (B220) eVolve™ 655. Total cells were used for analysis.