60031AZ

antibody from STEMCELL Technologies Inc

Targeting: PTPRC CD45, GP180, LCA, T200

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Recommended by provider)

Functional assay (Recommended by provider)

Antibody Data

Product number

60031AZ

Provider

STEMCELL Technologies Inc

Product name

Anti-Mouse Ly-6G Antibody, Clone 1A8, APC

Antibody type

Monoclonal

Description

The 1A8 antibody reacts with Ly-6G, a 21 - 25 kDa GPI-anchored protein, which together with the structurally related Ly-6C protein comprises the granulocyte receptor-1 antigen (Gr-1). Gr-1 is expressed on monocytes, neutrophils and subsets of macrophages, plasmacytoid dendritic cells, and T cells. Monocytes in the bone marrow transiently express Gr-1 during development and the expression level is strongly correlated with granulocyte differentiation and maturation. In the periphery, Gr-1 is found predominantly on neutrophils and is a useful marker for these cells. The 1A8 antibody binds specifically to Ly-6G, whereas another commonly used antibody, clone RB6-8C5, binds to both Ly-6G and Ly-6C. It has been reported that the 1A8 antibody detects Ly-6G-expressing granulocytes in peripheral blood, whereas the RB6-8C5 antibody also binds to Ly-6C-expressing lymphocytes, monocytes, and dendritic cells. This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse Neutrophil Enrichment Kit (Catalog #19762).

Reactivity

Mouse

Host

Mouse

Isotype

IgG

Vial size

100 µg

Storage

2 - 8°C