OAAB09847

antibody from Aviva Systems Biology

Targeting: ESR1 ER-alpha, Era, ESR, NR3A1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

OAAB09847

Provider

Aviva Systems Biology

Proper citation

Aviva Systems Biology Cat#OAAB09847, RRID:AB_2630423

Product name

ESR1/ER antibody - center region

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Vial size

400ul

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

ESR1/ER Antibody (Center) (Cat. #OAAB09847) western blot analysis in MCF-7 cell line lysates (35µg/lane).This demonstrates the ESR1/ER antibody detected the ESR1/ER protein (arrow).ESR1 is strongly supported by BioGPS gene expression data to be expressed in MCF7

Immunocytochemistry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of ESR1/ER Antibody (Center)(Cat#OAAB09847) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Immunohistochemistry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

ESR1/ER Antibody (Center) (Cat. #OAAB09847)immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ESR1/ER Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.