GTX62423

antibody from GeneTex

Targeting: ESR1 ER-alpha, Era, ESR, NR3A1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX62423

Provider

GeneTex

Proper citation

GeneTex Cat#GTX62423, RRID:AB_10621190

Product name

Estrogen Receptor alpha antibody [EPR4097]

Antibody type

Monoclonal

Reactivity

Human, Canine

Host

Rabbit

Storage

Store at -20 ¢XC. Stable for 12 months from date of receipt.

References

Submitted references

GREB1 isoforms regulate proliferation independent of ERα co-regulator activities in breast cancer.

Haines CN, Braunreiter KM, Mo XM, Burd CJ
Endocrine-related cancer 2018 Jul;25(7):735-746

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Estrogenic compounds reduce influenza A virus replication in primary human nasal epithelial cells derived from female, but not male, donors.

Peretz J, Pekosz A, Lane AP, Klein SL
American journal of physiology. Lung cellular and molecular physiology 2016 Mar 1;310(5):L415-25

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Targeting Estrogen Receptor Signaling with Fulvestrant Enhances Immune and Chemotherapy-Mediated Cytotoxicity of Human Lung Cancer.

Hamilton DH, Griner LM, Keller JM, Hu X, Southall N, Marugan J, David JM, Ferrer M, Palena C
Clinical cancer research : an official journal of the American Association for Cancer Research 2016 Dec 15;22(24):6204-6216

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis on (A) MCF-7 (B) T47-D and (C) HeLa cell lysates using anti-ER alpha RabMAb (cat. # GTX62423).

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

WB analysis of (1) MCF-7 and (2) T47-D lysates (20 £gg per lane) using Estrogen Receptor alpha antibody [EPR4097] at a dilution of 1:2000.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ICC/IF analysis of MCF-7 cells using Estrogen Receptor alpha antibody [EPR4097] at a dilution of 1:200 (green) and DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IHC-P analysis of human breast tissue using Estrogen Receptor alpha antibody [EPR4097] at a dilution of 1:250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Counterstained with Hematoxylin.