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LearnPA5-21478
antibody from Invitrogen Antibodies
Targeting: PARP3
ADPRT3, ADPRTL3, ARTD3, hPARP-3, IRT1, pADPRT-3
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
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- Product number
- PA5-21478 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-PARP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, HeLa, A375, mouse heart, PC-12. Predicted reactivity: Mouse (81%), Rat (82%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The poly(ADP-ribose)-dependent chromatin remodeler Alc1 induces local chromatin relaxation upon DNA damage.
Sellou H, Lebeaupin T, Chapuis C, Smith R, Hegele A, Singh HR, Kozlowski M, Bultmann S, Ladurner AG, Timinszky G, Huet S
Molecular biology of the cell 2016 Dec 1;27(24):3791-3799
Molecular biology of the cell 2016 Dec 1;27(24):3791-3799
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PARP3 using A) 30 µg A431 whole cell lysate (B) 30 µg HeLa whole cell lysate and C) 30 µg A375 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PARP3 polyclonal antibody (Product # PA5-21478) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PARP3 using 50 µg of mouse heart lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PARP3 polyclonal antibody (Product # PA5-21478) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PARP3 using 30 µg PC-12 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PARP3 polyclonal antibody (Product # PA5-21478) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PARP3 was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PARP3 Polyclonal Antibody (Product # PA5-21478) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PARP3 in paraformaldehyde-fixed HeLa cells using a PARP3 polyclonal antibody (Product # PA5-21478) at a 1:500 dilution.
