- UM500036 - OriGene ERBB2 antibody

Submitted references Development of a highly specific HER2 monoclonal antibody for immunohistochemistry using protein microarray chips.

Qi L, Zhou L, Lu M, Yuan K, Li Z, Wu G, Huang X, Shen Y, Zhao M, Fu W, Chu B, Wang G, Ren F, Ma D, Chen J
Biochemical and biophysical research communications 2017 Mar 4;484(2):248-254

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Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Western blot analysis of cell lysates of HEK293 transfected with ERBB2 cDNA (Left lane) or untransfected (Right lane) by using ERBB2 UltraMAB (UM500036, clone UMAB36, 1:2000).
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERBB2 monoclonal antibody (Clone UMAB36) at 1:500.
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Anti-ERBB2 mouse monoclonal antibody (UM500036, clone UMAB36, 1:100) immunofluorescent staining of COLO205 cells.
Validation comment: IF

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Anti-ERBB2 mouse monoclonal antibody (UM500036, clone UMAB36, 1:100) immunofluorescent staining of MCF7 cells.
Validation comment: IF

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-ERBB2mouse monoclonal antibody. (Clone UMAB36, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of breast tissue (HER2+++; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) using anti-ERBB2 mouse monoclonal antibody. (Clone UMAB36, dilution 1:100; heat-induced epit
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of four paraffin-embedded 293T cell lines transfected with EGFR, ERBB2, ERBB3, or ERBB4 using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, UM500036)(1:500). The image shows only ERBB2 transfected cell line lights up with anti-ERBB2 clone UMAB36 ?showing no cross-reactivity to the other EGFR family members.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of FFPE HER2+ breast cancer; using heat-induced epitope retrieval HIER at 120C for 3min with TEE buffer pH9.0, mouse monoclonal antibody anti-ERBB2 clone UMAB36 was used at 1ug/mL. Strong membrane staining seen in tumor cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERBB2 mouse monoclonal antibody (Clone UMAB36). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-ERBB2 (Clone UMAB36) very specifically recognizes ERBB2 antigen on OriGene protein microarray chip.
Validation comment: 10K-CHIP

UM500036

Antibody data