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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB3034 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3034, RRID:AB_1574739
- Product name
- ERBB2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ERBB2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Characterization of a new allele of the human ERBB2 gene by allele-specific competition hybridization.
Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor.
Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene.
Ehsani A, Low J, Wallace RB, Wu AM
Genomics 1993 Feb;15(2):426-9
Genomics 1993 Feb;15(2):426-9
Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor.
Yamamoto T, Ikawa S, Akiyama T, Semba K, Nomura N, Miyajima N, Saito T, Toyoshima K
Nature 1986 Jan 16-22;319(6050):230-4
Nature 1986 Jan 16-22;319(6050):230-4
Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene.
Coussens L, Yang-Feng TL, Liao YC, Chen E, Gray A, McGrath J, Seeburg PH, Libermann TA, Schlessinger J, Francke U
Science (New York, N.Y.) 1985 Dec 6;230(4730):1132-9
Science (New York, N.Y.) 1985 Dec 6;230(4730):1132-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of ERBB2 polyclonal antibody (Cat # PAB3034) in SK-BR-3 cell lysate. ERBB2 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with ERBB2 polyclonal antibody (Cat # PAB3034) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 cells using ERBB2 polyclonal antibody (Cat # PAB3034)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
