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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- TA503443 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA503443, RRID:AB_2622397
- Product name
- ERBB2 mouse monoclonal antibody, clone OTI4F10 (formerly 4F10)
- Antibody type
- Monoclonal
- Description
- ERBB2 mouse monoclonal antibody, clone OTI4F10 (formerly 4F10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ERBB2
- Isotype
- IgG
- Antibody clone number
- OTI4F10
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Open-gate mutants of the mammalian proteasome show enhanced ubiquitin-conjugate degradation.
Choi WH, de Poot SA, Lee JH, Kim JH, Han DH, Kim YK, Finley D, Lee MJ
Nature communications 2016 Mar 9;7:10963
Nature communications 2016 Mar 9;7:10963
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERBB2 (RC212583, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERBB2.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-ERBB2 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-ERBB2 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-ERBB2 mouse monoclonal antibody (TA503443) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERBB2(RC212583).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503443)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503443)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503443)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC212583 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERBB2 antibody(TA503443), and then analyzed by flow cytometry.
- Validation comment
- FC
