151204

antibody from BioLegend

Targeting: MKI67 MIB-1, PPP1R105

Immunocytochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

151204

Provider

BioLegend

Proper citation

BioLegend Cat#151204, RRID:AB_2566800

Product name

Alexa Fluor® 488 anti-mouse/human Ki-67

Antibody type

Monoclonal

Antigen

E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinant protein.

Reactivity

Human, Mouse

Host

Rat

Conjugate

Green dye

Antibody clone number

11F6

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

C57BL/6 mouse frozen intestine section was fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% triton X-100 for ten minutes, and blocked with 5% FBS plus 5% rat/mouse serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of Ki-67 (clone 11F6) Alexa Fluor® 488 (green) and 5 µg/ml of E-cadherin (clone DECMA-1) Alexa Fluor® 594 (red) in 5% FBS overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 20X objective.

Conjugate

Green dye