M00254-3

antibody from Boster Biological Technology

Targeting: MKI67 MIB-1, PPP1R105

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

M00254-3

Provider

Boster Biological Technology

Product name

Anti-Human Ki67 Antibody

Antibody type

Polyclonal

Description

Anti-Human Ki67 Antibody tested in WB, IF, ICC, IHC, reactive to Human, Rat, Mouse

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100ul

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

Storage for short term at 4℃ recommended, for longer term at -20℃, minimize freeze/thaw cycles

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of equal amounts of cell lysates using rabbit pAb to Ki-67 M00254-3, dilution 1:10,000, (green): [1] protein standard (red), [2] HeLa cells, [3] HEK293 cells. Strong double bands above 250kDa correspond to the two major Ki-67 isoforms of molecular weight 345kDa and 395kDa. Smaller proteolytic fragments of these isoforms are also detected on the blot.

Additional image